期刊文献

Genome-Wide Identification and Drought-Responsive Functional Analysis of the GST Gene Family in Potato (Solanum tuberosum L.) 收藏

全基因组鉴定和对马铃薯中GST基因家族的干旱响应功能分析（索拉纳姆结核L.）
原文求助 发布源
作者
Ningfan Shi [1];Youfang Fan [2];Wei Zhang [3];Zhijia Zhang [4];Zhuanfang Pu [5];Zhongrun Li [6];Lijun Hu [7];Zhenzhen Bi [8];Panfeng Yao (https://orcid.org/0000-0003-1291-3555) [9];Yuhui Liu [10];Zhen Liu [11];Jiangping Bai [12];Chao Sun [13];
作者单位
[1]College of Agronomy/State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China; [2]College of Agronomy/State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China; [3]College of Agronomy/State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China; [4]College of Agronomy/State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China; [5]College of Agronomy/State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China; [6]College of Agronomy/State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China; [7]College of Agronomy/State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China; [8]College of Agronomy/State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China; [9]College of Agronomy/State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China; [10]College of Agronomy/State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China; [11]College of Agronomy/State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China; [12]College of Agronomy/State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China; [13]College of Agronomy/State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China;
发布日期
2025-2-19
页码
239
DOI
10.3390/antiox14020239
来源信息
Antioxidants ISSN：2076-3921, 2025年, 14卷, 2期, 239页
摘要
Glutathione S-transferases (GSTs) play crucial roles in crop stress tolerance through protection against oxidative damage. In this study, we conducted genome-wide identification and expression analysis of the GST gene family in the autotetraploid potato cultivar Cooperative-88 (C88) using bioinformatic approaches. We identified 366 GST genes in the potato genome, which were classified into 10 subfamilies. Chromosomal mapping revealed that StGSTs were distributed across all 12 chromosomes, with 13 tandem duplication events observed in three subfamilies. Analysis of protein sequences identified 10 conserved motifs, with motif 1 potentially representing the GST domain. Analysis of cis-acting elements in the StGSTs promoter regions suggested their involvement in stress response pathways. RNA-seq analysis revealed that most StGSTs responded to both drought stress and DNA demethylation treatments. Quantitative PCR validation of 16 selected StGSTs identified four members that showed strong responses to both treatments, with distinct expression patterns between drought-tolerant (QS9) and drought-sensitive (ATL) varieties. Transient expression assays in tobacco demonstrated that these four StGSTs enhanced drought tolerance and may be regulated through DNA methylation pathways, though the precise mechanisms require further investigation. These findings provide a theoretical foundation for understanding the response and epigenetic regulation of potato GST genes under drought stress.
摘要译文
谷胱甘肽S-转移酶（GSTS）通过防止氧化损伤在作物胁迫耐受性中起着至关重要的作用。在这项研究中，我们使用生物信息学方法对自动二倍体马铃薯品种合作 - 88（C88）中GST基因家族进行了全基因组鉴定和表达分析。我们确定了马铃薯基因组中的366个GST基因，这些基因被分类为10个亚家族。染色体图显示，STGST分布在所有12个染色体上，在三个亚科中观察到13个串联重复事件。蛋白质序列的分析确定了10个保守基序，其中基序1代表GST结构域。对STGSTS启动子区域中顺式作用元件的分析表明，它们参与了压力反应途径。RNA-seq分析表明，大多数STGST都对干旱胁迫和DNA脱甲基化处理做出了反应。对16个选定的STGST的定量PCR验证确定了四个对两种治疗的反应强烈的成员，在耐旱（QS9）和对干旱敏感（ATL）品种之间具有不同的表达模式。烟草中的瞬时表达测定表明，这四个STGST增强了干旱耐受性，并且可以通过DNA甲基化途径调节，尽管精确的机制需要进一步研究。这些发现为理解在干旱压力下对马铃薯GST基因的反应和表观遗传调节提供了理论基础。
Ningfan Shi [1];Youfang Fan [2];Wei Zhang [3];Zhijia Zhang [4];Zhuanfang Pu [5];Zhongrun Li [6];Lijun Hu [7];Zhenzhen Bi [8];Panfeng Yao (https://orcid.org/0000-0003-1291-3555) [9];Yuhui Liu [10];Zhen Liu [11];Jiangping Bai [12];Chao Sun [13];. Genome-Wide Identification and Drought-Responsive Functional Analysis of the GST Gene Family in Potato (Solanum tuberosum L.)[J]. Antioxidants, 2025,14(2): 239