摘要
To determine the optimum fermentation condition, the 50:50 feed mixture of both crushed corn and wet brewer’s grain were anaerobically fermented at various conditions. Three supplementation levels(0, 2%, and 5%) of molasses, five supplementation levels(0, 0.1%, 0.5%, 1.0%, and 2.0%) of yeast and five different incubation temperatures(4, 10, 30, 40 and 50) were tested to determine the optimum fermentation conditions. During fermentation, alcohol concentration, live yeast cell number (LYCN) and pH values of the mixture were analyzed. Alcohol concentrations of the fermented feed mixtures were proportionally increased with increasing level of molasses supplementation. After 24hr fermentation, alcohol concentrations at 0.1%, 0.5%, 1.0% and 2.0% of yeast supplementation was 1.9%, 2.4%, 3.1% and 3.1%, respectively. These results indicate a proportional increase of alcohol concentration with the increasing supplementation of yeast (P<0.05) up to 1%, thereafter showing plateau at 2% supplementation. LYCN was relatively high with more than 0.5% yeast supplementation, whereas the value was significantly low(P<0.05) at 0.1% yeast supplementation. Alcohol production was significantly higher at 30 and 40 than other incubation temperatures (P<0.05), with the highest concentration at 30. pH was rapidly decreased until 24 hours of incubation with steeper decrease at lower temperatures(P<0.05). Based on there results, optimum fermentation conditions for the alcoholic-fermented feed production could be achieved with 5% supplementation of molasses, 1% supplementation of yeast, and 24hr long incubation at 30.
摘要译文
为了确定最佳发酵条件，将碎玉米和湿啤酒糟的50:50进料混合物在各种条件下进行厌氧发酵。三种补充水平（0％，2％和5％）的糖蜜，五种补充水平（0,0.1％，0.5％，1.0％和2.0％）的酵母和五种不同的孵化温度（4,10,30,40）测试50）以确定最佳发酵条件。在发酵过程中，分析了酒精浓度，活酵母细胞数（LYCN）和混合物的pH值。发酵饲料混合物的酒精浓度随着糖蜜补充水平的增加而成比例地增加。发酵24小时后，酵母补充的0.1％，0.5％，1.0％和2.0％的酒精浓度分别为1.9％，2.4％，3.1％和3.1％。这些结果表明，随着酵母补充量的增加（P <0.05），酒精浓度成比例增加至1％，此后在2％补充时显示出平台期。 LYCN相对较高，酵母补充超过0.5％，而在0.1％酵母补充时，该值显着低（P <0.05）。酒精产量在30和40时显着高于其他培养温度（P <0.05），最高浓度为30℃。pH值迅速下降，直至孵育24小时，在较低温度下急剧下降（P <0.05）。基于该结果，可以通过添加5％的糖蜜，1％的酵母补充物和30小时的24小时长的孵育来实现酒精发酵饲料生产的最佳发酵条件。
Kim, C.H.; Park, B.K.; Ohh, S.J.; Sung, K.I.; Kim, H.S.; Hong, B.J.; Shin, J.S.;. Determination of Optimum Fermentation Condition for Alcohol-Fermented Feeds with Wet Brewer's Grains[J]. Journal of Animal Science and Technology. (Text mainly in Korean; Summaries in English), 2005,47(4): 607-614