摘要
Chysobalanus icaco L. (C. icaco) is a plant that is native to tropical America and Africa. It is also found in the southeast region of Mexico, where it is used as food and to treat certain diseases. This study aimed to carry out a phytochemical analysis of an aqueous extract of C. icaco seed (AECS), including its total phenol content (TPC), total flavonoid content (TFC), and condensed tannins (CT). It also aimed to examine the antioxidant and metal-ion-reducing potential of the AECS in vitro, as well as its toxicity and anti-inflammatory effect in mice. Antioxidant and metal-ion-reducing potential was examined by inhibiting DPPH, ABTS, and FRAP. The acute toxicity test involved a single administration of different doses of the AECS (0.5, 1, and 2 g/kg body weight). Finally, a single administration at doses of 150, 300, and 600 mg/kg of the AECS was used in the carrageenan-induced model of subplantar acute edema. The results showed that the AECS contained 124.14 ± 0.32 mg GAE, 1.65 ± 0.02 mg EQ, and 0.910 ± 0.01 mg of catechin equivalents/g dried extract (mg EC/g de extract) for TPC, TFC and CT, respectively. In the antioxidant potential assays, the values of the median inhibition concentration (IC50) of the AECS were determined with DPPH (0.050 mg/mL), ABTS (0.074 mg/mL), and FRAP (0.49 mg/mL). Acute toxicity testing of the AECS revealed no lethality, with a median lethal dose (LD50) value of >2 g/kg by the intragastric route. Finally, for inhibition of acute edema, the AECS decreased inflammation by 55%, similar to indomethacin (59%, p > 0.05). These results demonstrated that C. icaco seed could be considered a source of bioactive molecules for therapeutic purposes due to its antioxidant potential and anti-inflammatory activity derived from TPC, with no lethal effect from a single intragastric administration in mice.
摘要译文
Chysobalanus iCaco L.（C。icaco）是一种是热带美国和非洲本地的植物。它也可以在墨西哥东南地区发现，在那里它被用作食物并治疗某些疾病。这项研究旨在对C. c. c. c.c。c.c。C.c。C.c。C.c。c.cotecor（AEC）进行植物化学分析，包括其总苯酚含量（TPC），总类黄酮含量（TFC）和凝结的单宁（CT）。它还旨在检查体外AEC的抗氧化剂和金属还原潜力，以及其在小鼠中的毒性和抗炎作用。通过抑制DPPH，ABT和FRAP检查了抗氧化剂和金属降低的潜力。急性毒性测试涉及不同剂量的AEC（0.5、1和2 g/kg体重）的单一给药。最后，用于150、300和600 mg/kg的AEC剂量的单一给药被用于carlageenan诱导的亚平态急性水肿的模型。结果表明，对于TPC，TFC和CT，AEC含有124.14±0.32 mg GAE，1.65±0.02 mg EQ和0.910±0.01 mg的儿茶素等效物（MG EC/G de提取物）（MG EC/G de提取物）。在抗氧化电位测定中，用DPPH（0.050 mg/ml），ABT（0.074 mg/ml）和FRAP（0.49 mg/ml）确定AEC的中值抑制浓度（IC50）的值。AEC的急性毒性测试没有表现出致命性，胃内途径的中位致死剂量（LD50）值> 2 g/kg。最后，为了抑制急性水肿，AEC的炎症降低了55％，类似于吲哚美辛（59％，p> 0.05）。这些结果表明，C. icaco种子可以被视为用于治疗目的的生物活性分子的来源，因为其抗氧化潜力和源自TPC的抗炎活性，而小鼠中单一的胃内给药没有致命作用。
Abel Arce-Ortiz (0000-0002-9711-6039) [1];Cristian Jiménez-Martínez (0000-0001-8921-9858) [2];Gabriel Alfonso Gutiérrez-Rebolledo (0000-0002-2189-7908) [3];Luis Jorge Corzo-Ríos (0000-0003-0987-7522) [4];Zendy Evelyn Olivo-Vidal (0000-0001-6242-7964) [5];Rosalva Mora-Escobedo (0000-0003-3197-1187) [6];Yair Cruz-Narváez [7];Xariss M. Sánchez-Chino (0000-0002-8599-8150) [8];. Evaluation of the Antioxidant and Anti-Inflammatory Activities and Acute Toxicity of Caco Seed (Chrysobalanus icaco L.) in Murine Models[J]. Molecules, 2024,29(14): 3243