期刊文献

Analysis of major androgen-regulated cDNA clones from the rat epididymis 收藏

大鼠附睾中主要雄激素调节的cDNA克隆的分析

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作者

Jane E.Walker[1];RoyJones[2];AlisonMoore[3];David W.Hamilton[3];LenHall[1];

作者单位

[a]Department of Biochemistry, University of Bristol, School of Medical Sciences, Bristol BS8 1TD, U.K.[b]Department of Molecular Embryology, Institute of Animal Physiology and Genetics Research, Babraham, Cambridge CB4, U.K.[c]Department of Cell Biology and Neuroanatomy, University of Minnesota, Minneapolis, MN 55455, U.S.A.[∗]Address for correspondence: Dr. Len Hall, Department of Biochemistry, University of Bristol, School of Medical Sciences, Bristol BS8 1TD, U.K..

关键词

Epididymis; Epididymal-specific genes; Hormonal regulation;

关键词译文

附睾;附睾特异性基因;荷尔蒙调节;

发布日期

12 November 1990

页码

61-68

DOI

10.1016/0303-7207(90)90205-M

来源信息

Molecular and Cellular Endocrinology ISSN：0303-7207, 1990年, 74卷, 1期, 61-68页

摘要

Four abundant cDNA clones have been isolated from a rat epididymal cDNA library. Northern blot analysis has shown that these clones partially encode 4.5 kb, 2.8 kb, 1.2 kb and 0.85 kb mRNAs and that their expression is not detectable in total RNA preparations from heart, kidney, liver or testis. Fourteen days after castration the levels of the 2.8 kb, 1.2 kb and 0.85 kb transcripts were greatly reduced whereas the 4.5 kb mRNA was undetectable. Subsequent treatment of castrated rats with testosterone for 1 day resulted in a complete restoration of the pre-castration steady-state levels of the 2.8 kb and 0.85 kb mRNAs, restoration of the 4.5 kb mRNA to 70% of pre-castration levels, and a slight over-induction of the 1.2 kb mRNA. Analyses of separate regions of the epididymal tract showed that expression of the 2.8 kb and 1.2 kb mRNAs increased towards the distal end of the epididymis, while the 4.5 kb and 0.85 kb transcripts were primarily synthesised in the caput region.

摘要译文

从大鼠附睾cDNA文库中分离出4个丰富的cDNA克隆。 Northern印迹分析显示这些克隆部分编码4.5kb，2.8kb，1.2kb和0.85kb mRNA，并且它们的表达在来自心脏，肾脏，肝脏或睾丸的总RNA制剂中是不可检测的。阉割后14天，2.8kb，1.2kb和0.85kb转录物的水平大大降低，而4.5kb mRNA不可检测。随后用睾酮治疗阉割大鼠1天，完全恢复2.8kb和0.85kb mRNA的预阉割稳态水平，将4.5kb mRNA恢复至70％的预阉割水平，并且轻微过度诱导1.2 kb mRNA。对附睾管道的不同区域的分析显示，2.8kb和1.2kb mRNA的表达朝向附睾的远端增加，而4.5kb和0.85kb的转录物主要在头部区域中合成。

Jane E.Walker[1];RoyJones[2];AlisonMoore[3];David W.Hamilton[3];LenHall[1];. Analysis of major androgen-regulated cDNA clones from the rat epididymis[J]. Molecular and Cellular Endocrinology, 1990,74(1): 61-68