摘要
Regulation of transcription within four well-defined regions of the DNA genome of the baculovirus Autographa californica nuclear polyhedrosis virus (AcNPV) was studied in a permissive lepidopteran cell line, Spodoptera frugiperda IPLB-SF-21 and a nonpermissive dipteran cell line, Drosophila melanogaster DL-1. Cycloheximide, an inhibitor of protein synthesis, was used to identify immediate early transcripts and aphidicolin, an effective inhibitor of DNA replication, was used to distinguish early and late transcripts in S. frugiperda cells. Immediate early transcripts were identified in the HindIII-K (85 to 87.5%), the HindIII-I/EcoRI-F (I/F from 35 to 37%) and the HindIII-P/EcoRI-P (p10 from 88.5 to 89.5%) regions of the genome. Late RNAs, defined by their sensitivity to aphidicolin as well as cycloheximide, overlapped the immediate early RNAs. Specific viral transcripts were synthesized in nonpermissiveD. melanogaster cells infected with AcNPV but late viral transcripts were not observed.
摘要译文
在允许的鳞翅目细胞系Spodoptera frugiperda IPLB-SF-21和不允许的双翅目细胞系Drosophila melanogaster DL中研究了杆状病毒苜蓿银纹夜蛾核多角体病毒（AcNPV）的DNA基因组的四个明确区域内的转录调节。 -1。环己酰亚胺（蛋白质合成的抑制剂）用于鉴定即刻早期转录物，并且aphidicolin（一种有效的DNA复制抑制剂）用于区分S.frugiperda细胞中的早期和晚期转录物。立即早期转录物在HindIII-K（85至87.5％），HindIII-I / EcoRI-F（I / F从35至37％）和HindIII-P / EcoRI-P（p10从88.5至89.5）中鉴定。 ％）基因组区域。晚期RNA由对蚜虫素和环己酰亚胺的敏感性定义，与早期的直接RNA重叠。在非常规D中合成特异性病毒转录物。用AcNPV感染的黑腹果蝇细胞但未观察到晚期病毒转录物。
William C.Rice[∗∗];Lois K.Miller;. Baculovirus transcription in the presence of inhibitors and in nonpermissive Drosophila cells[J]. Virus Research, 1986,6(2): 155-172