摘要
Microsatellite markers are widely used in aquaculture for genetic analysis and breeding programs, but challenges such as segregation distortion and allelic instability can impact their effectiveness in parentage verification and inheritance studies. This study evaluated 15 microsatellite loci in seven experimental olive flounder (Paralichthys olivaceus) families bred through 1:1 full-sibling crosses, assessing their utility for accurate parentage and inheritance stability. Parentage assignments were conducted within an expanded pool of 647 candidate parents (including the actual 14 parents), encompassing both closely related and moderately distant individuals. Despite increased genetic diversity, assignments maintained a high accuracy rate (99.6%), demonstrating marker robustness. Differences in delta values highlighted the influence of genetic backgrounds on assignment clarity, with some groups showing reduced distinctiveness in the expanded pool. Segregation distortion was observed at certain loci, deviating from Mendelian inheritance, likely due to meiotic drive and post-zygotic selection. These findings underscore the need for empirical validation of microsatellite loci for stable inheritance and reliable parentage in diverse breeding programs, especially with genetically similar spawners.
摘要译文
微卫星标志物被广泛用于水产养殖中，用于遗传分析和育种计划，但是诸如隔离失真和等位基因不稳定性之类的挑战可以影响其在父母验证和继承研究中的有效性。这项研究评估了在七个实验性橄榄比目（Paralichthys olivaceus）家族中通过1：1的全同胞十字繁殖的家族的15个微卫星基因座，评估了它们的效用，以进行准确的父母和继承稳定性。父母的分配是在647个候选父母（包括实际的14个父母）的扩大池中进行的，包括密切相关和中等距离的人。尽管遗传多样性增加，但分配仍保持高精度率（99.6％），表明标记鲁棒性。增量值的差异突出了遗传背景对分配清晰度的影响，其中一些组显示出扩展的池中的独特性降低。在某些基因座观察到隔离失真，偏离了门德尔的遗传，这可能是由于减数分裂驱动和循环后选择引起的。这些发现强调了对微卫星基因座基因座的经验验证的必要性，以在不同的育种计划中，尤其是在遗传上相似的产卵中，对稳定的遗传和可靠的亲属需要。
Songhyun Gwon; Eunjeong Kim; Wonse Lee; Jisung Han; Yoonkwon Nam. Parentage Verification and Segregation Distortion Patterns of Microsatellite Markers in Olive Flounder (Paralichthys olivaceus) Full-Sib Families[J]. Animals, 2025,15(2): 176