摘要
The uterine cervix at term undergoes histomorphological changes that resemble an inflammatory process. The aim of this study was to better characterize these changes, describing the temporal and spatial pattern of macrophages and mast cells (MC) distribution in the uterine cervix and assessing whether both cells exert a coordinated action on angiogenesis. Macrophages and MC were identified by immunohistochemistry in cervical tissue from cycling, pregnant and postpartum rats. In order to inhibit MC degranulation, pregnant rats were injected with disodium cromoglycate. The expression of vascular endothelial growth factor (VEGF) by macrophages was also evaluated. Results showed that macrophage density increased towards parturition and declined at postpartum, whereas MC density showed an inverse pattern. Interestingly, disodium cromoglycate-treated rats showed an increased number of macrophages. VEGF expression in macrophages was detected neither in control nor in treated animals; however, a coordinated action between MC and macrophages on angiogenesis could not be excluded. The present study provides a detailed mapping of macrophage and MC densities and distribution in the rat uterine cervix. Moreover, an association between macrophages and MC along pregnancy is shown, and evidence that macrophage density in the rat cervix is modulated by MC degranulation is presented.
摘要译文
足月的子宫颈经历类似炎症过程的组织形态学变化。这项研究的目的是更好地描述这些变化，描述子宫颈中巨噬细胞和肥大细胞（MC）分布的时间和空间模式，并评估两种细胞是否对血管生成发挥协调作用。通过免疫组织化学在来自循环，怀孕和产后大鼠的宫颈组织中鉴定巨噬细胞和MC。为了抑制MC脱粒，给怀孕的大鼠注射色甘酸二钠。还评估了巨噬细胞对血管内皮生长因子（VEGF）的表达。结果显示，巨噬细胞密度朝分娩增加，并在产后下降，而MC密度呈反向模式。有趣的是，色甘酸二钠处理的大鼠显示出巨噬细胞数量增加。在对照组和治疗组中均未检测到巨噬细胞中VEGF的表达;然而，不能排除MC和巨噬细胞之间在血管生成方面的协调作用。本研究提供了大鼠子宫颈巨噬细胞和MC密度及分布的详细图谱。此外，显示了巨噬细胞和MC在怀孕期间的关联，并且提出了通过MC脱粒调节大鼠子宫颈中巨噬细胞密度的证据。
Verónica L.Bosquiazzo[1];MilenaDurando[1];JorgelinaVarayoud;Jorge G.Ramos;Horacio A.Rodríguez;MónicaMuñoz-de-Toro;Enrique H.Luque;. Macrophage density in the pregnant rat uterine cervix is modulated by mast cell degranulation[J]. Journal of Reproductive Immunology, 2005,65(2): 147-158