期刊文献

Effects of Active Dry Yeast Supplementation in In Vitro and In Vivo Nutrient Digestibility, Rumen Fermentation, and Bacterial Community 收藏

在体外和体内营养消化率，瘤胃发酵和细菌群落中补充活性干酵母的影响

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作者

作者单位

[1]State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, Lanzhou University, Lanzhou 730020, China; [2]State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, Lanzhou University, Lanzhou 730020, China; [3]State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, Lanzhou University, Lanzhou 730020, China; [4]Animal Husbandry Work Station of Ningxia, Yingchuan 750002, China; [5]Centro Regional de Investigación Carillanca, Instituto de Investigaciones Agropecuarias INIA, Vilcún 4880000, Chile; [6]State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, Lanzhou University, Lanzhou 730020, China; [7]State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, Lanzhou University, Lanzhou 730020, China; [8]Aksu Tycoon Feed Co., Ltd., Aksu 843000, China; [9]Aksu Tycoon Feed Co., Ltd., Aksu 843000, China; [10]Tecon Pharmaceutical Co., Ltd., Suzhou 215000, China;

发布日期

2024-10-9

页码

2916

DOI

10.3390/ani14192916

来源信息

Animals ISSN：2076-2615, 2024年, 14卷, 19期, 2916页

摘要

This study assessed the impact of active dry yeast (ADY) on nutrient digestibility and rumen fermentation, using both in vitro and in vivo experiments with lambs. In vitro, ADYs were incubated with rumen fluid and a substrate mixture to assess gas production, pH, volatile fatty acid (VFA) profiles, and lactate concentration. In vivo, Hu lambs were randomly assigned to five dietary treatments: a control group and four groups receiving one of two dosages of either Vistacell or Procreatin7. Growth performance, nutrient digestibility, rumen fermentation parameters, and bacterial community composition were measured. Pro enhanced the propionate molar proportion while it decreased the n-butyrate molar proportion. Vis reduced the lactate concentration in vitro. In the in vivo experiment, Vis increased the propionate molar proportion and the Succinivibrionaceae_UCG-001 abundance while it decreased the n-butyrate molar proportion and the Lachnospiraceae_ND3007 abundance. Additionally, Vis showed a greater impact on improving the NDF digestibility and total VFA concentration in vivo compared to Pro. Overall, the effects of ADYs on rumen fermentation were found to vary depending on the specific ADY used, with Vis being the most suitable for lamb growth. It was observed that Vis promoted propionate fermentation and Succinivibrionaceae_UCG-001 abundance at the expense of reduced n-butyrate fermentation and Lachnospiraceae_ND3007 abundance. Importantly, differences were noted between the outcomes of the in vitro and in vivo experiments concerning the effects of ADYs on rumen fermentation, highlighting the need for caution when generalizing batch culture results to the in vivo effects of ADYs.

摘要译文

这项研究使用羔羊的体外和体内实验评估了活性干酵母（ADY）对营养消化率和瘤胃发酵的影响。在体外，将ADYS与瘤胃液和底物混合物一起孵育，以评估气体产生，pH，挥发性脂肪酸（VFA）谱和乳酸浓度。在体内，HU羔羊被随机分配给五种饮食治疗：对照组和四个接受Vistacell或Procreatin7的两种剂量之一。测量了生长性能，营养消化率，瘤胃发酵参数和细菌群落组成。Pro增强了丙酸酯摩尔比例，同时降低了N-丁酸摩尔比例。观察会在体外降低乳酸浓度。在体内实验中，VIS增加了丙酸酯摩尔比例和琥珀曲纤维酸-UCG-001丰度，同时降低了N-丁酸摩尔摩尔比例和lachnospiraceae_nd3007的丰度。此外，与Pro相比，VIS对改善体内NDF消化率和总VFA浓度的影响更大。总体而言，发现ADY对瘤胃发酵的影响因使用的特定ADY而异，VIS最适合羔羊生长。据观察，VIS促进了丙酸发酵和琥珀酸-1ucg-001的丰度，以减少n-丁酸发酵和lachnospiraceae_nd3007的丰度为代价。重要的是，在体外的结果和体内实验中，关于ADYS对瘤胃发酵的影响之间的差异，突出了在将批处理培养概括为ADYS的体内影响时需要谨慎的差异。

Haitao Liu (0009-0000-9678-1160) [1];Fei Li [2];Zhiyuan Ma (0000-0003-1185-5621) [3];Miaomiao Ma [4];Emilio Ungerfeld (0000-0002-5422-5462) [5];Zhian Zhang [6];Xiuxiu Weng (0000-0002-4832-5643) [7];Baocang Liu [8];Xiaoyu Deng [9];Liqing Guo [10];. Effects of Active Dry Yeast Supplementation in In Vitro and In Vivo Nutrient Digestibility, Rumen Fermentation, and Bacterial Community[J]. Animals, 2024,14(19): 2916