摘要
Our previous research has demonstrated that the spinal cord undergoes epigenetic and molecular alterations following non-severe burn injury (BI). However, the primary somatosensory cortex (S1), crucial for pain perception, remains unexplored in this context. Here, we investigated transcriptomic alterations in the S1 cortex of mice subjected to BI or formalin application (FA) to the hind paw, utilizing RNA sequencing (RNA-seq) one hour after injury. RNA-seq identified 1116 differentially expressed genes (DEGs) in BI and 136 DEGs in formalin-induced inflammatory pain. Notably, 82.4% of DEGs in BI and 32.4% in FA were downregulated. A total of 42 upregulated and 17 downregulated overlapping DEGs were identified, indicating significant differences in the cortical processing of pain based on its origins. Gene Ontology analysis reveals that BI upregulated mitochondrial functions and ribosome synthesis, whereas axon guidance, synaptic plasticity, and neurotransmission-related processes were downregulated. By contrast, formalin treatment mainly impacted metabolic processes. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis highlights the significance of retrograde endocannabinoid signaling (REC) in the response to burn injury. These findings demonstrate that transcriptomic remodeling in the S1 cortex is dependent on the sensory modality and suggest that the REC network is activated during acute pain responses following BI.
摘要译文
我们先前的研究表明，脊髓在非严重性烧伤后经历表观遗传和分子改变（BI）。然而，在这种情况下，对疼痛感至关重要的主要体感皮质（S1）仍未探索。在这里，我们研究了受伤后一小时使用RNA测序（RNA-Seq），研究了接受BI或福尔马林施用（FA）的小鼠S1皮层的转录组改变。RNA-seq在BI中鉴定出1116个差异表达的基因（DEG），在福尔马林诱导的炎症性疼痛中鉴定了136摄氏度。值得注意的是，BI的DEG的82.4％和FA中的32.4％被下调。总共确定了42个上调和17个下调的重叠DEG，这表明疼痛的皮质处理基于其起源的显着差异。基因本体分析表明，BI上调线粒体功能和核糖体合成，而轴突引导，突触可塑性和与神经传递相关的过程被下调。相比之下，福尔马林治疗主要影响代谢过程。基因和基因组（KEGG）途径分析的京都百科全书突出了逆行内源性内源性信号传导（REC）在对烧伤损伤反应中的重要性。这些发现表明，S1皮层中的转录组重塑取决于感觉方式，并表明在BI后急性疼痛反应中激活REC网络。
Virág Erdei; Zoltán Mészár; Angelika Varga. The Burning Pain Transcriptome in the Mouse Primary Somatosensory Cortex[J]. International Journal of Molecular Sciences, 2025,26(8): 3538