摘要
There is considerable interest in the possibility of using insect hormones or hormone regulatory proteins in conjunction with microbial delivery systems as a means of controlling insect pests. We have employed Autographa californica nuclear polyhedrosis virus (AcMNPV) to express a Manduca sexta (L.) cDNA encoding eclosion hormone (EH) to explore the effects this hormone might have during infection in larvae. AcMNPV naturally blocks molting of its insect host by expression of an ecdysteroid UDP-glucosyltransferase (egt) gene. EH-expressing viruses which either possessed or did not possess an intact egt gene were tested. M. sexta EH produced by recombinant viruses elicited eclosion behavior when injected into pharate Spodoptera frugiperda (J. E. Smith) pupae. The presence of the EH gene reduced the infectivity of the virus slightly in neonate S. frugiperda. Expression of the EH gene during infection of late instar S. frugiperda larvae had neither a positive nor a negative effect on the ability of AcMNPV to limit insect feeding or hasten death. No differences in behavior were observed in larvae infected with EH-recombinant versus wild-type virus.
摘要译文
对于将昆虫激素或激素调节蛋白与微生物递送系统结合使用作为控制害虫的手段的可能性存在相当大的兴趣。我们采用Autographa californica核多角体病毒（AcMNPV）来表达编码羽化激素（EH）的Manduca sexta（L.）cDNA，以探索这种激素在幼虫感染过程中可能产生的影响。 AcMNPV通过表达蜕皮类固醇UDP-葡糖基转移酶（egt）基因而天然阻断其昆虫宿主的蜕皮。测试了具有或不具有完整egt基因的表达EH的病毒。由重组病毒产生的M. sexta EH在注入噬菌体草地贪夜蛾（Spodoptera frugiperda）时引起羽化行为（J. E.史密斯）蛹。 EH基因的存在在新生S. frugiperda中略微降低了病毒的感染性。在晚期的S. frugiperda幼虫感染期间表达EH基因对AcMNPV限制昆虫摄食或加速死亡的能力既没有正面影响也没有负面影响。在用EH-重组体与野生型病毒感染的幼虫中没有观察到行为差异。
RussEldridge[∗];David R.O'Reilly[†][1];Lo is K.Miller[†];. Efficacy of a baculovirus pesticide expressing an eclosion hormone gene[J]. Biological Control, 1992,2(2): 104-110