期刊文献

Synthesis of Infectious Bacteriophages in an E. coli-based Cell-free Expression System 收藏

基于大肠杆菌的无细胞表达系统中感染性噬菌体的合成

原文求助发布源

作者

Mark Rustad; Allen Eastlund; Ryan Marshall; Paul Jardine; ; Vincent Noireaux

作者单位

[1]School of Physics and Astronomy, University of MinnesotaFind articles by Mark Rustad[2]Department of Diagnostic and Biological Sciences and Institute for Molecular Virology, University of MinnesotaFind articles by Allen Eastlund[1]School of Physics and Astronomy, University of MinnesotaFind articles by Ryan Marshall[2]Department of Diagnostic and Biological Sciences and Institute for Molecular Virology, University of MinnesotaFind articles by Paul Jardine[1]School of Physics a

关键词

Bioengineering, Issue 126, Cell-free transcription-translation (TXTL), bacteriophage, E. coli, plaque assay, phage amplification, phage genome purification, synthetic biology

关键词译文

生物工程，问题126，无细胞转录 - 翻译（TXTL），噬菌体，大肠杆菌，斑块测定，噬菌体扩增，噬菌体基因组净化，合成生物学

发布日期

2017

DOI

10.3791/56144

来源信息

Journal of Visualized Experiments : JoVE ISSN：1940-087X, 2017年, 126期

摘要

A new generation of cell-free transcription-translation (TXTL) systems, engineered to have a greater versatility and modularity, provide novel capabilities to perform basic and applied sciences in test tube reactions. Over the past decade, cell-free TXTL has become a powerful technique for a broad range of novel multidisciplinary research areas related to quantitative and synthetic biology. The new TXTL platforms are particularly useful to construct and interrogate biochemical systems through th

摘要译文

设计的新一代无细胞转录 - 翻译（TXTL）系统，用于具有更大的通用性和模块化，提供了新的能力，以在试管反应中执行基本和应用的科学。在过去十年中，无细胞TXTL已成为与定量和合成生物学相关的广泛新型多学科研究领域的强大技术。新的TXTL平台特别适用于通过TH构建和询问生化系统

Mark Rustad; Allen Eastlund; Ryan Marshall; Paul Jardine; ; Vincent Noireaux. Synthesis of Infectious Bacteriophages in an E. coli-based Cell-free Expression System[J]. Journal of Visualized Experiments : JoVE, 2017(126)