摘要
Polycystic ovary syndrome (PCOS) is a common endocrine-related cause of infertility in women and has an unknown etiology. Studies have shown that rhamnocitrin (Rha) exhibits positive effects on the reproductive system. This study investigated Rha’s antifibrotic effects on PCOS rats and revealed its underlying mechanisms. Female SD rats were randomized into 4 groups ( n = 8 , each); the control group received tea oil by intraperitoneal injection and 1% w / v CMC by oral gavage; the PCOS group received letrozole (1 mg/kg); the PCOS+Rha group received letrozole and Rha (5 mg/kg); the PCOS+Met group received letrozole and Met (265 mg/kg) for 21 days. At the study end, Rha treatment restored letrozole-induced alterations in the relative ovarian weights, body weight, and relative weights of uterine and visceral adipose tissues. Histological observation showed that Rha ameliorates ovarian structure and fibrosis in PCOS. Administration of Rha reduced letrozole-induced metabolic dysfunction by ameliorating the levels of TC, TG, and HDL-C in the PCOS rats. Rha treatment also modulated the serum levels of sex hormones, which decreased T, E2, and LH and increased FSH in PCOS rats. In addition, Rha treatment modulated insulin resistance and increased gene expression of antioxidant enzymes (Cat, Sod2, Gpx3, Mgst1, Prdx3, Gsta4, Gsr, and Sod1) in the ovaries of the PCOS rats. Finally, Rha treatment appeared to increase the activity of PPAR-γ and inhibit the TGF-β1/Smad pathway in the ovaries of the PCOS rats. Our findings suggest that Rha significantly ameliorated metabolic disturbances and ovarian fibrosis in the PCOS rats. Rha perhaps is an effective compound for preventing ovarian fibrosis in the future.
摘要译文
多囊卵巢综合征（PCOS）是女性不育症的常见原因，病因未知。研究表明，鼠李糖蛋白（RHA）对生殖系统表现出积极的影响。这项研究调查了RHA对PCOS大鼠的抗纤维化作用，并揭示了其潜在的机制。雌性SD大鼠被随机分为4组（n = 8，每个）；对照组通过腹膜内注射接受茶油，口服饲料1％w / v CMC；PCOS组接受了LeTrozole（1 mg/kg）；PCOS+RHA组接受了LeTrozole和RHA（5 mg/kg）；PCOS+MET组接收了LeTrozole并MET（265 mg/kg）21天。在研究结束时，RHA处理恢复了letrozole诱导的相对卵巢重量，体重和子宫和内脏脂肪组织的相对重量的改变。组织学观察结果表明，RHA可以改善PCOS中的卵巢结构和纤维化。通过改善PCOS大鼠的TC，TG和HDL-C的水平，施用RHA会降低letrozole诱导的代谢功能障碍。RHA治疗还调节了性激素的血清水平，可降低T，E2和LH，并增加PCOS大鼠的FSH。此外，RHA处理调节了抗氧化剂酶（CAT，SOD2，GPX3，MGST1，PRDX3，GSTA4，GSR和SOD1）的胰岛素抵抗和增加的基因表达。最后，RHA处理似乎增加了PPAR-γ的活性，并抑制了PCOS大鼠卵巢中的TGF-β1/SMAD途径。我们的发现表明，RHA显着改善了PCOS大鼠的代谢障碍和卵巢纤维化。RHA也许是预防卵巢纤维化的有效化合物。
Yanyuan Zhou [1];Huan Lan [2];Zhewen Dong [3];Wanying Li [4];Bo Qian [5];Zhen Zeng [6];Wen He [7];Jia-Le Song (0000-0001-6269-5846) [8];. Rhamnocitrin Attenuates Ovarian Fibrosis in Rats with Letrozole-Induced Experimental Polycystic Ovary Syndrome[J]. Oxidative Medicine and Cellular Longevity, 2022,2022(1): 1-18