期刊文献

Superfecundation induction by intrauterine insemination with different frozen-thawed canine semen and parentage test using microsatellite analysis 收藏

使用微卫星分析通过宫腔内人工授精不同冻融犬精液和亲子鉴定诱导超级诱导
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作者
Lee, Ji Hye; Kim, Keun Jung; Choi, Seon A; Li, Xiaoxia; Kim, Eun Young; Oh, Hyun Ju; Lee, Byeong Chun; Kim, Hye Jin; Park, Byung Kwon; Kim, Min Kyu;
关键词
frozen-thawed semen;intrauterine insemination;microsatellite;superfecundation;
关键词译文
冻融精液;宫腔内人工授精;微卫星;超级受精;
页码
285-290
来源信息
Korean Journal of Veterinary Research ISSN：2466-1384, 2009年, 49卷, 4期, 285-290页
摘要
This study was performed to investigate the possibility of superfecundation by surgical intrauterine artificial insemination in dogs of confirmed genetic pedigree. Artificial insemination was performed on 3 days after ovulation with spermatozoa. Five puppies were delivered on 60 days after insemination. The ratio of the number of newborns to the number of corpora lutea was 83.3% (5/6). Parentage analysis with 10 canine-specific microstatellite markers demonstrated that one puppy was genetically relative to the sire-A family and four puppies were genetically relative to the sire-B. The present study demonstrated that two kinds of puppies with different genetic pedigree can be produced by surgical uterine insemination of semen of individual dog into each uterine horn of a bitch.
摘要译文
本研究旨在探讨通过手术宫内人工授精对已确认遗传谱系的犬进行超级分娩的可能性。在用精子排卵后3天进行人工授精。授精后60天分娩五只幼犬。新生儿数量与黄体数量的比率为83.3％（5/6）。使用10只犬特异性微卫星标记的亲子关系分析表明，一只小狗在遗传上相对于父系-A家族，并且四只小狗在遗传上相对于父系-B。本研究表明，通过将个别犬的精液外科子宫授精进入母犬的每个子宫角，可以产生两种具有不同遗传谱系的幼犬。
Lee, Ji Hye; Kim, Keun Jung; Choi, Seon A; Li, Xiaoxia; Kim, Eun Young; Oh, Hyun Ju; Lee, Byeong Chun; Kim, Hye Jin; Park, Byung Kwon; Kim, Min Kyu;. Superfecundation induction by intrauterine insemination with different frozen-thawed canine semen and parentage test using microsatellite analysis[J]. Korean Journal of Veterinary Research, 2009,49(4): 285-290