期刊文献

Artificial Insemination with Frozen Canine Semen Using Vaginal Endoscope 收藏

阴道内窥镜人工冷冻冷冻犬精液
摘要
Artificial insemination with frozen canine semen using vaginal endoscope was performed in a two years old Golden Retriever to investigate the feasibility of this technique. The bitch was in her second estrus. Blood progesterone concentration was evaluated every two days to decide the timing of insemination since vaginal bleeding was observed. Insemination was performed twice in 48 hours interval on the fourth and sixth posterior to LH surge, respectively. The volume of frozen semen injected was 1.5 ml $(1{\times}10^{8}$ sperms/1ml of semen). The bitch was pregnant and she whelped a female pup on sixty one days after first insemination.
摘要译文
在两年大的金毛猎犬中使用阴道内窥镜对冷冻犬精液进行人工授精,以研究该技术的可行性。 ch子在她的第二次发情期。由于观察到阴道出血,每两天评估一次血液中的孕激素浓度,以决定授精的时间。在LH激增后的第四次和第六次,每48小时进行一次授精。注射的冷冻精液量为1.5 ml(1 {—次} 10 ^ {8} $精子/ 1 ml精液)。母狗怀孕了,在第一次受精后的第六十一天,她为一只母幼崽提供了帮助。
Artificial Insemination with Frozen Canine Semen Using Vaginal Endoscope[J]. Journal of Veterinary Clinics 한국임상수의학회지, 1997,14(2): 297-300