摘要
Purpose: Horse racing may cause stress-induced physiological changes and tissue damage in horses, but the changes in miRNA expression, protein expression, and metabolic substances in the plasma exosomes of the Yili horse after racing are still unclear. This study detected miRNA, protein expression, and metabolic substances in the plasma exosomes of Yili horses before and after competition, providing new insights for post-race recovery and care of Yili horses. Method: Eight three-year-old Yili horses that had undergone training were selected as the research subjects, with four horses that had not competed as the control group and four horses that had participated in the competition for half an hour as the training group. Extract whole blood and separate plasma from two groups of horses, and then extract plasma exosomes; MiRNAs, proteins, and metabolites in extracellular vesicles were detected and analyzed using miRNAomics, proteomics, and metabolomics. P Result: After the competition, the levels of miRNAs related to the cytoplasm and nucleus in Yili horse plasma exosomes increased, and miRNAs related to the transcription and transcriptional regulation of biological processes significantly increased. The levels of proteins related to the cytoplasm and nucleus also increased, and the levels of proteins related to cell signaling function increased, carbohydrates and their metabolites were significantly reduced. Conclusions: The competition process causes significant changes in the miRNA, proteomics, and metabolomics of plasma exosomes in the Yili horses, which are mainly related to metabolic regulation.
摘要译文
目的：赛马可能会导致马匹的生理变化和组织损伤，但是赛车后，miRNA表达，蛋白质表达和代谢物质的变化仍然不清楚。这项研究检测到竞争前和之后的YILI马血浆外泌体中的miRNA，蛋白质表达和代谢物质，为赛后恢复和YILI马匹的护理提供了新的见解。方法：八匹三岁的YILI马被选为研究对象，四匹马没有作为对照组竞争，四匹马参加了比赛，他们参加了半个小时的训练小组。从两组马中提取全血和分离血浆，然后提取血浆外泌体；使用mirnaomics，蛋白质组学和代谢组学检测并分析细胞外囊泡中的miRNA，蛋白质和代谢物。P结果：竞争结束后，与YILI马血浆外泌体中与细胞质和核有关的miRNA水平增加，与生物学过程的转录和转录调控有关的miRNA显着增加。与细胞质和细胞核相关的蛋白质水平也增加，与细胞信号功能相关的蛋白质水平增加，碳水化合物及其代谢产物的水平显着降低。结论：竞争过程导致miRNA，蛋白质组学和YILI马血浆外泌体的代谢组学的显着变化，这主要与代谢调节有关。
Xinxin Yuan; Xinkui Yao; Yaqi Zeng; Jianwen Wang; Wanlu Ren; Tongliang Wang; Xueyan Li; Lipin Yang; Xixi Yang; Jun Meng. The Impact of the Competition on miRNA, Proteins, and Metabolites in the Blood Exosomes of the Yili Horse[J]. Genes, 2025,16(2): 224