摘要
Gammaretroviral and lentiviral vectors pseudotyped with vesicular stomatitis virus glycoprotein G (VSV-G) have been used for stable gene transfer because of their broad host range and high mechanical strength. In the present study, an expression plasmid for chimeric VSV-G, consisting of a ZZ fragment derived from Staphylococcal protein A fused to the N-terminus of VSV-G (ZZ-VSV-G), was constructed to produce viral vectors capable of antibody-dependent gene transduction. Gammaretroviral (based on mouse stem cell virus, MSCV) and lentiviral (based on human immunodeficiency virus type 1, HIV-1) vectors pseudotyped with ZZ-VSV-G were produced without the loss of antibody-binding activity. The production of infectious viral particles was promoted by the addition of an expression plasmid for native VSV-G and antibody-dependent gene transduction was achieved using plates coated with antibodies. This system may be useful for the genetic transduction of cells expressing specific proteins on their surface, and for screening of antibodies specific for cell surface receptors.
摘要译文
用水疱性口炎病毒糖蛋白G（VSV-G）假型化的γ-逆转录病毒和慢病毒载体已被用于稳定的基因转移，因为它们具有广泛的宿主范围和高机械强度。在本研究中，构建了嵌合VSV-G的表达质粒，其由源自与VSV-G的N-末端融合的葡萄球菌蛋白A的ZZ片段（ZZ-VSV-G）组成，以产生能够进行的病毒载体。抗体依赖性基因转导。产生Gamma逆转录病毒（基于小鼠干细胞病毒，MSCV）和用ZZ-VSV-G假型化的慢病毒（基于人类免疫缺陷病毒1型，HIV-1）载体，而不丧失抗体结合活性。通过添加天然VSV-G的表达质粒促进感染性病毒颗粒的产生，并且使用涂有抗体的平板实现抗体依赖性基因转导。该系统可用于在其表面上表达特定蛋白质的细胞的遗传转导，以及用于筛选对细胞表面受体特异的抗体。
YujiroKameyama;YoshinoriKawabe;AkiraIto;MasamichiKamihira;. Antibody-dependent gene transduction using gammaretroviral and lentiviral vectors pseudotyped with chimeric vesicular stomatitis virus glycoprotein[J]. Journal of Virological Methods, 2008,153(1): 49-54