期刊文献

Antioxidant and Photoprotective Activities of 3,4-Dihydroxybenzoic Acid and (+)-Catechin, Identified from Schima argentea Extract, in UVB-Irradiated HaCaT Cells 收藏

3,4-二羟基苯甲酸和（+） - 儿茶素的抗氧化剂和光保护活性，从schima argentea提取

原文求助发布源

作者

Qi He [1];Yu-Pei Chen (https://orcid.org/0000-0001-9108-5756) [2];Junhao Li [3];Hongtan Wu [4];Fangfang Chen [5];Mingyu Li [6];Chun Wu [7];

作者单位

[1]The School of Public Health, Fujian Medical University, Fuzhou 350122, China; [2]The School of Public Health, Fujian Medical University, Fuzhou 350122, China; [3]Key Laboratory of Functional and Clinical Translational Medicine, Fujian Province University, Xiamen Medical College, Xiamen 361023, China; [4]The School of Public Health and Medical Technology, Xiamen Medical College, Xiamen 361023, China; [5]The School of Public Health and Medical Technology, Xiamen Medical College, Xiamen 361023, China; [6]The School of Public Health, Fujian Medical University, Fuzhou 350122, China; [7]Key Laboratory of Functional and Clinical Translational Medicine, Fujian Province University, Xiamen Medical College, Xiamen 361023, China;

发布日期

2025-2-19

页码

241

DOI

10.3390/antiox14020241

来源信息

Antioxidants ISSN：2076-3921, 2025年, 14卷, 2期, 241页

摘要

In traditional Chinese medicine, the root bark and leaves of Schima argentea are utilized to treat dysentery, parasitic infections, and digestive disorders. In this study, the n-butanol extract of S. argentea (NBA) exhibited potent antioxidant properties, protecting HaCaT cells from UVB-induced damage, and was abundant in phenolic and flavonoid compounds. Using UPLC-QTOF-MS analysis, several antioxidants within NBA were identified. Among these, 3,4-dihydroxybenzoic acid, (+)-catechin, and procyanidin B2 effectively reduced ROS levels after 1 h post-UVB treatment (225 mJ/cm2). Notably, all three compounds significantly decreased the phosphorylation of p38 and JNK in a dose-dependent manner. Additionally, the cell survival rate of these compounds was assessed after 12 h post-UVB treatment (225 mJ/cm2). Both 3,4-dihydroxybenzoic acid and (+)-catechin significantly prevented UVB-induced apoptosis in HaCaT cells, as evidenced by MTT, Hoechst, Calcein/PI staining, and flow cytometry analyses. Proteomic analysis revealed that 3,4-dihydroxybenzoic acid achieved photoprotection by downregulating c-Fos and Jun and modulating cell cycle proteins, while (+)-catechin promoted cell repair through the PI3K-Akt and Wnt signaling pathways. These results demonstrated that both compounds can directly absorb UVB, scavenge ROS, and provide cell photoprotection by modulating multiple signaling pathways. The n-butanol extract of S. argentea holds promising potential for future medical applications.

摘要译文

在传统中药中，阿根廷西马的根树皮和叶子用于治疗痢疾，寄生虫感染和消化系统疾病。在这项研究中，阿根廷链霉菌（NBA）的N-丁醇提取物表现出有效的抗氧化特性，可保护HACAT细胞免受UVB诱导的损伤，并且在酚类和类黄酮化合物中很丰富。使用UPLC-QTOF-MS分析，确定了NBA内的几种抗氧化剂。其中，3,4-二羟基苯甲酸，（+） - 儿茶素和procyanidin b2在治疗后1小时（225 MJ/cm2）后有效地降低了ROS水平。值得注意的是，所有三种化合物都以剂量依赖性方式显着降低了p38和JNK的磷酸化。此外，在UVB治疗后12小时（225 MJ/cm2）后，评估了这些化合物的细胞存活率。如MTT，Hoechst，Calcein/Pi染色和流式细胞术分析所证明的，3,4-二羟基苯甲酸和（+） - 儿茶素都显着阻止了HACAT细胞中UVB诱导的凋亡。蛋白质组学分析表明，3,4-二羟基苯甲酸通过下调C-FOS和JUN和调节细胞周期蛋白来实现光保护，而（+） - 儿茶素通过PI3K-AKT和WNT信号通路促进了细胞修复。这些结果表明，两种化合物都可以直接吸收UVB，清除ROS，并通过调节多个信号通路来提供细胞的光保护。S. Argentea的N-丁醇提取物具有未来医疗应用的有希望的潜力。

Qi He [1];Yu-Pei Chen (https://orcid.org/0000-0001-9108-5756) [2];Junhao Li [3];Hongtan Wu [4];Fangfang Chen [5];Mingyu Li [6];Chun Wu [7];. Antioxidant and Photoprotective Activities of 3,4-Dihydroxybenzoic Acid and (+)-Catechin, Identified from Schima argentea Extract, in UVB-Irradiated HaCaT Cells[J]. Antioxidants, 2025,14(2): 241