期刊文献

Analysis of the Primary Pathogenic Bacteria in Abscess Disease of Musk Deer Using Metagenomic Approaches 收藏

使用宏基因组方法分析麝香鹿脓肿的主要致病细菌
原文求助 发布源
作者
Jingyao Hu [1];Xian An [2];Pengcheng Yang [3];Rongzeng Tan [4];Taoyue Chen [5];Jiatong Chen [6];Yifan Tao [7];Xuxin Li [8];Runbin Sun [9];Shouyun Zhang [10];Shuqiang Liu [11];Liangliang Yang [12];
作者单位
[1]College of Nature Conservation, Beijing Forestry University, Beijing 100107, China; [2]College of Nature Conservation, Beijing Forestry University, Beijing 100107, China; [3]College of Nature Conservation, Beijing Forestry University, Beijing 100107, China; [4]College of Nature Conservation, Beijing Forestry University, Beijing 100107, China; [5]College of Nature Conservation, Beijing Forestry University, Beijing 100107, China; [6]College of Nature Conservation, Beijing Forestry University, Beijing 100107, China; [7]College of Nature Conservation, Beijing Forestry University, Beijing 100107, China; [8]Zhangzhou Pien Tze Huang Pharmaceutical Co., Ltd., Zhangzhou 600436, China; [9]Huailai Zhiyangtianbao Technical Development Co., Ltd., Huailai, Zhangjiakou 075400, China; [10]Huailai County Forestry Bureau, Huailai, Zhangjiakou 075400, China; [11]College of Nature Conservation, Beijing Forestry University, Beijing 100107, China; [12]Key Laboratory of Forest Protection of National Forestry and Grassland Administration, Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing 100091, China;
发布日期
2025-4-11
页码
1105
DOI
10.3390/ani15081105
来源信息
Animals ISSN：2076-2615, 2025年, 15卷, 8期, 1105页
摘要
Abscesses are among the diseases affecting the survival of captive musk deer and are difficult to identify in their early stages. In this study, metagenomic sequencing, 16S rRNA sequencing, and paraffin sectioning were used to analyze the microbiota in the abscess musk deer pus group (AMP), abscess musk deer oral group (AMO), and healthy musk deer oral group (HMO) to compare the differences in microbiota in musk deer. By detecting differences in the oral microbiota through throat swabs, we aimed to monitor the early onset of abscess disease to facilitate timely intervention and treatment. The results showed that the alpha diversity of HMO microbiota was significantly higher than that of the AMP and AMO samples. Beta diversity results indicated that there were significant differences in the bacterial communities of HMO and AMO samples, and no significant difference was found between AMO and AMP samples. A taxonomic analysis of the bacterial species indicated that differences between HMO and AMP groups were found in the Fusobacterium and Trueperella species. Fusobacterium and Trueperella were the main pathogenic bacteria responsible for the occurrence of abscess diseases in forest musk deer in this study. Furthermore, the appearance of Fusobacterium and Trueperella in the oral cavity can serve as biomarkers for the early diagnosis of abscess disease in musk deer.
摘要译文
脓肿是影响圈养麝香生存的疾病之一，在早期阶段很难识别出脓肿。在这项研究中，使用元基因组测序，16S rRNA测序和石蜡切片分析脓肿的Musk Musk Deer Pus组（AMP），脓肿的Musk Musk Deer群（AMO）和健康的Musk Deer Oral Group（HMO）中的微生物群，以比较Musk Deerer的差异。通过通过喉咙拭子检测口腔微生物群的差异，我们旨在监测脓肿疾病的早期发作，以促进及时的干预和治疗。结果表明，HMO微生物群的α多样性显着高于AMP和AMO样品。β多样性结果表明，HMO和AMO样品的细菌群落存在显着差异，并且在AMO和AMP样品之间没有发现显着差异。对细菌物种的分类学分析表明，在梭杆菌和Trueperella物种中发现了HMO和AMP组之间的差异。在这项研究中，梭杆菌和trueperella是负责在森林麝香鹿中发生脓肿疾病的主要致病细菌。此外，口腔中梭杆菌和毛甲虫的出现可以作为麝香鹿早期诊断出脓肿疾病的生物标志物。
Jingyao Hu [1];Xian An [2];Pengcheng Yang [3];Rongzeng Tan [4];Taoyue Chen [5];Jiatong Chen [6];Yifan Tao [7];Xuxin Li [8];Runbin Sun [9];Shouyun Zhang [10];Shuqiang Liu [11];Liangliang Yang [12];. Analysis of the Primary Pathogenic Bacteria in Abscess Disease of Musk Deer Using Metagenomic Approaches[J]. Animals, 2025,15(8): 1105