期刊文献

Vitamin C Inhibits Scale Drop Disease Virus Infectivity by Targeting Nrf2 to Reduce Ferroptosis 收藏

维生素C通过靶向NRF2抑制量表疾病病毒感染性降低降水量

原文求助发布源

作者

作者单位

[1]State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China; [2]State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China; [3]State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China; [4]State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China; [5]State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China;

发布日期

2025-5-10

页码

576

DOI

10.3390/antiox14050576

来源信息

Antioxidants ISSN：2076-3921, 2025年, 14卷, 5期, 576页

摘要

Scale drop disease virus (SDDV) poses an escalating threat to global aquaculture, prompting an urgent need for research. Our study found that SDDV infection upregulates genes related to iron, oxidative stress, and lipid metabolism, causing iron overload, reactive oxygen species (ROS) accumulation, and ultimately ferroptosis. Among the tested antioxidants, vitamin C (VC) demonstrated the most potent inhibitory effect in mandarin fish, reducing SDDV-induced mortality by 37.5%. qPCR and IFA results showed that VC effectively suppressed SDDV infection; decreased ROS, lipid peroxidation (LPO), and iron levels; and enhanced glutathione peroxidase 4 (GPX4) expression in infected cells. Mechanistically, VC’s inhibitory effect was reversed by the nuclear factor erythroid 2-related factor 2 (Nrf2) inhibitor ML-385, indicating an Nrf2-dependent pathway. VC promoted Nrf2 nuclear translocation and activated downstream antioxidant genes. Moreover, VC modulated inflammation by regulating pro- and anti-inflammatory factors. These findings suggest VC as a promising therapeutic for SDDV infection.

摘要译文

鳞片疾病病毒（SDDV）对全球水产养殖构成了不断升级的威胁，迫使人们迫切需要研究。我们的研究发现，SDDV感染上调了与铁，氧化应激和脂质代谢相关的基因，导致铁超载，活性氧（ROS）积累以及最终导致铁毒性。在测试的抗氧化剂中，维生素C（VC）在普通话中表现出最有效的抑制作用，使SDDV诱导的死亡率降低了37.5％。QPCR和IFA结果表明，VC有效抑制了SDDV感染。降低ROS，脂质过氧化（LPO）和铁水平；并增强了感染细胞中的谷胱甘肽过氧化物酶4（GPX4）的表达。从机械上讲，VC的抑制作用被核因子2相关因子2（NRF2）抑制剂ML-385逆转，表明NRF2依赖性途径。VC促进了NRF2核转运并激活下游抗氧化剂基因。此外，VC通过调节促疾病和抗炎因素调节炎症。这些发现表明VC是SDDV感染的有希望的治疗性。

Jiaming Chen (https://orcid.org/0009-0004-5859-4806) [1];Yuting Fu [2];Shaoping Weng [3];Jianguo He (https://orcid.org/0000-0003-2460-7061) [4];Chuanfu Dong (https://orcid.org/0000-0001-6276-7993) [5];. Vitamin C Inhibits Scale Drop Disease Virus Infectivity by Targeting Nrf2 to Reduce Ferroptosis[J]. Antioxidants, 2025,14(5): 576