期刊文献

Yeast Cell Cultivation of Produce Active Dry Yeast with Improved Viability 收藏

具有提高的生存力的活性干酵母的酵母细胞培养

原文求助发布源

作者

Kim, Geun;Kim, Jae-Yun;

关键词

active dry yeast;dehydration;survival rate;culture condition

关键词译文

活性干酵母脱水存活率培养条件

发布日期

1999.10.01

页码

561-565

来源信息

KSBB Journal ISSN：1225-7117, 1999年, 14卷, 5期, 561-565页

摘要

Optimum conditions for vacuum-drying ad cultivation of yeast cells for the production of active dry yeast were examined. At lower temperature, more drying time was required to dry the yeast pellet to reach the desirable water content(8%). Optimum temperature of vaccum oven and time for drying was 63$^{\circ}C$ and 90 min, respectively. Optimum medium composition for flask culture using cane molasses as the substrate were 0.25% sugar, 0.013% $K_2$HPO$_4$, 0.1% $K_2$HPO$_4$. and 0.125% (NH$_4$)$_2$SO$_4$. Culture temperature $25^{\circ}C$ gave the highest survival rate of dired yeast. After finishing fed-batch culture and the culture was left in the fermentor without adding any sugar or nutrient, survival of the dried yeast harvested from the fermentor increased to 86.0% after 36 hr. It was also observed that the yeast cells with higher budding rates showed lower survival rate.

摘要译文

检查了用于真空干燥和酵母培养以生产活性干酵母的最佳条件。在较低的温度下，需要更多的干燥时间来干燥酵母颗粒以达到所需的水分含量（8％）。真空烘箱的最佳温度和干燥时间分别为63℃和90分钟。使用甘蔗糖蜜作为底物进行烧瓶培养的最佳培养基组成是0.25％的糖，0.013％的K_2 $ HPO_4 $，0.1％的K_2 $ HPO_4 $。和0.125％（NH $ _4 $）$ _ 2 $ SO $ _4 $。培养温度$ 25 ^ {-circ} C $使干酵母的存活率最高。分批补料培养完成后，将培养物留在发酵罐中而不添加任何糖或营养，36小时后，从发酵罐中收获的干燥酵母的存活率提高到86.0％。还观察到出芽率较高的酵母细胞显示出较低的存活率。

Kim, Geun;Kim, Jae-Yun;. Yeast Cell Cultivation of Produce Active Dry Yeast with Improved Viability[J]. KSBB Journal, 1999,14(5): 561-565