摘要
The liver of hens bears a very heavy workload in handling lipogenesis as well as carbohydrate, protein, and other nutrient metabolism to maintain a high laying rate. D-glucuronolactone (DGL) is a naturally occurring chemical metabolite produced from glucose and exhibits a hepatoprotective effect as a food ingredient. This study aimed to evaluate the effect of DGL on production performance, egg quality, and liver health in laying hens during their peak period. Four hundred and eighty Hy-Line brown hens (aged 42 weeks), with comparable high-laying performance, were randomly assigned to four groups, with each group consisting of eight replicates. The control group was fed a basal diet, while the treatment groups were supplemented with DGL at 70, 140, and 280 mg/kg in the basal diet, respectively. The results indicated that DGL supplementation significantly increases the laying rate and egg mass of hens (p < 0.05). The brown color of the eggshell was significantly deepened by DGL administration (p < 0.05). Additionally, DGL supplementation significantly decreased the serum levels of aspartate aminotransferase and γ-glutamyl transpeptidase (p < 0.05). The liver index, liver fat, and triglyceride levels in the liver had also significantly decreased (p < 0.05). Liver histopathological results showed that the control group exhibited yellow coloration with large gaps and numerous vacuolar cells, while DGL supplementation improved the compactness of liver parenchyma. Antioxidant activity was enhanced, showing a significant increase in the activity of total antioxidant capacity and catalase in the liver (p < 0.05). The inflammatory response was alleviated, with a significant decrease in expressions of interleukin-β, tumor necrosis factor-α, interferon-α, interleukin-6, and interleukin-8 genes in the liver (p < 0.05). Moreover, DGL supplementation significantly reduced cell apoptosis-related genes, including factor-related apoptosis, Caspase 7, and BCL2 associated X protein in the liver (p < 0.05). Overall, these findings indicate that dietary DGL enhanced the laying performance of hens by maintaining liver health, with the best results achieved at a dose of 280 mg/kg in this trial.
摘要译文
母鸡的肝脏在处理脂肪生成以及碳水化合物，蛋白质和其他营养代谢方面具有很大的工作量，以保持高铺设率。D-葡萄糖醛酮（DGL）是由葡萄糖产生的天然化学代谢物，表现出作为食物成分的肝保护作用。这项研究旨在评估DGL在高峰时段产生母鸡生产性能，卵质量和肝脏健康的影响。四百八十个Hy-Line棕色母鸡（42周），具有可比的高层表现，随机分配给四组，每组由八个重复组成。对照组为基础饮食喂食，而治疗组分别以70、140和280 mg/kg的基础饮食补充了DGL。结果表明，补充DGL会显着增加母鸡的产卵率和卵子质量（p <0.05）。DGL给药可显着加深蛋壳的棕色（P <0.05）。此外，补充DGL可显着降低天冬氨酸氨基转移酶和γ-谷氨酰基转肽酶的血清水平（P <0.05）。肝脏中的肝脏指数，肝脏脂肪和甘油三酸酯水平也显着降低（P <0.05）。肝组织病理学结果表明，对照组表现出具有较大间隙和众多液泡细胞的黄色，而DGL补充剂则改善了肝实质的紧凑性。抗氧化活性得到了增强，显示肝脏中总抗氧化能力和过氧化氢酶的活性显着增加（p <0.05）。炎症反应得到缓解，肝脏白介素-β，肿瘤坏死因子-α，Interferon-α，白介素-6和白介素-8基因的表达显着降低（p <0.05）。此外，补充DGL可显着降低与细胞凋亡相关的基因，包括与因子相关的凋亡，caspase 7和Bcl2相关的X蛋白（p <0.05）。总体而言，这些发现表明，饮食DGL通过维持肝脏健康增强了母鸡的铺设性能，在这项试验中，最佳效果以280 mg/kg的剂量获得。
Yiru Shen (https://orcid.org/0000-0002-7263-1632) [1];Zhiqiang Miao [2];Yuqi Zheng [3];Yuanyang Dong (https://orcid.org/0000-0001-6967-3121) [4];Miaomiao Han (https://orcid.org/0000-0002-4806-8368) [5];Chenxuan Huang [6];Rui Bai [7];Chengqiang Xia [8];Shourong Shi (https://orcid.org/0000-0002-8011-2883) [9];Jianhui Li [10];. D-Glucuronolactone Supplementation Enhances Production Performance, Eggshell Quality, and Liver Health in Laying Hens[J]. Animals, 2025,15(9): 1317