摘要
A 3 year-old female Afghan hound came to the Veterinary Referral Hospital of the College of Veterinary Medicine, Seoul National University for artificial insemination (AI) with frozen semen. In order to inseminate, semen was frozen in USA 3 years ago. Frozen semen was sent by air from Santiago to Seoul for AI. The stud died 2 years ago, so we could only use a limited amount of frozen semen in that estrus cycle. The number of total motile spermatozoa was and the total volume was 1.2 ml. The frozen spermatozoa were thawed in water for 8 sec, which were then deposited at the bilateral uterine horns by a surgical method. The number of corpus luteum was 6. Sixty days after artificial insemination resulted in the birth of 4 puppies, all of which are alive and healthy.
摘要译文
一名3岁的阿富汗雌性猎犬来到首尔国立大学兽医学院兽医转诊医院进行人工授精（AI）和冷冻精液。为了受精，3年前在美国冻结了精液。冷冻精液通过空气从圣地亚哥送到首尔进行人工智能。螺柱在2年前死亡，因此我们只能在发情周期中使用有限量的冷冻精液。总运动精子的数量是，总体积是1.2毫升。将冷冻的精子在水中解冻8秒，然后通过手术方法将其沉积在双侧子宫角上。黄体数量为6.人工授精后60天导致4只幼犬出生，所有这些幼犬都活着并且健康。
Kim, Hye Jin; Oh, Hyun Ju; Jang, Goo; Yoon, Jung Hee; Lee, Hyung Suk; Kim, Sang Cheol; Hwang, Cheol Young; Kim, Dae Yong; Kim, Min Kyu;. Successful pregnancy following surgical intrauterine insemination using frozen semen in Afghan hound dog[J]. Korean Journal of Veterinary Research, 2007,47(1): 135-138