摘要
Hantaan virus-like particles (VLPs) have been produced and purified by co-expressing Hantaan virus nucleocapsid (N) protein and glycoproteins (Gn and Gc) in baculovirus insect cell systems. The potential of VLPs as a vaccine was evaluated by comparing the humoral immune response and neutralizing antiserum induced by the purified VLP and the recombinant baculovirus bearing the Hantaan virus glycoproteins alone. After immunization of C57BL/6 mice, Hantaan VLPs efficiently induced both hantaan virus-specific humoral immune response and neutralizing antibodies. Hantaan VLPs from baculovirus expression system also protected mice from HTNV challenge. These data provide information important to the development of Hantaan virus-like particles using the baculovirus-insect cell system as a potential vaccine for the prevention of Hantavirus infection.
摘要译文
汉坦病毒样颗粒（VLP）是通过在杆状病毒昆虫细胞系统中共表达汉坦病毒核衣壳蛋白（N）和糖蛋白（Gn和Gc）而产生和纯化的。通过比较纯化的VLP和仅携带汉坦病毒糖蛋白的重组杆状病毒诱导的体液免疫反应和中和抗血清，评估了VLP作为疫苗的潜力。免疫C57BL / 6小鼠后，汉坦病毒VLP有效诱导汉坦病毒特异性的体液免疫应答和中和抗体。来自杆状病毒表达系统的汉坦VLP也保护小鼠免受HTNV攻击。这些数据提供了对于开发汉坦病毒（如使用杆状病毒的颗粒）昆虫细胞系统作为预防汉坦病毒感染的潜在疫苗至关重要的信息。
Xiaoxia Dai[a][b]Can Jian[a][b] NaLi[a][b]Xiaofeng Wang[a][b]Yu Dai[a][b]Dexin Li[c]. Production and characterization of Hantaan virus-like particles from baculovirus expression system[J]. Biochemical Engineering Journal, 2019,152