摘要
This study evaluated the effects of kaempferol (KAE), and vitamin E (VE) on the performance, reproductive hormones, and the composition of the cecum and uterus microbiota in late-laying hens. A total of 192 49-week-old Jinghong No. 1 laying hens were randomly divided into four groups, with six replicates in each group and eight laying hens in each replicate, pre-reared for one week and formally tested for ten weeks. The CON group was fed basal diets, the VE group, the KAE group, and the KAE + VE group were fed a basal diet to which was added 0.2 g/kg VE, 0.4 g/kg KAE, and 0.2 g/kg VE + 0.4 g/kg KAE, respectively. The results are as follows. Compared to the CON group, the VE group, the KAE group, and the KAE + VE group significantly increased the egg production rate, average daily egg weight and significantly decreased the feed-to-egg ratio. The VE + KAE group significantly improved the Haugh unit. The VE group, the KAE group, and the KAE + VE group considerably enhanced the eggshell strength, eggshell relative weight, eggshell thickness, yolk color, and relative yolk weight. The serum E2 and LH levels of the KAE group and the KAE + VE group and the serum FSH levels of the KAE + VE group were significantly higher. In the ovary, the KAE group and the KAE + VE group’s ESR1 gene expression levels were significantly higher, and the KAE + VE group’s FSHR gene expression levels were markedly higher. In the uterus, the KAE group and the KAE + VE group’s ESR1 gene expression levels were dramatically higher, and the KAE + VE group’s ESR2 and FSHR gene expression levels were significantly higher. 16S rRNA gene sequencing revealed a significant aggregation of cecum and uterus colonies in the Beta diversity PCoA. In the cecum, Firmicutes, Bacteroidetes, and WPS-2 were the dominant phylums. In the uterus, the Firmicutes, Proteobacteria, and Bacteroidetes were the dominant phylums. The KAE + VE group’s F/B was significantly higher at the phylum level than in the CON group and the VE group. In summary, the addition of VE and KAE to the diet can improve the production performance of late-laying hens, increase the content of reproductive hormones, and stabilize the cecal and uterus microbiota, which may be related to the hormone and microbiota linkage of the gut–uterus axis.
摘要译文
这项研究评估了Kaempferol（KAE）和维生素E（VE）对晚期母鸡中盲肠和子宫菌群的性能，生殖激素和子宫组成的影响。总共将192个49周龄的第1胎母鸡分为四组，每组中有6个重复，每组重复的八个育雏，预先续签一周，并正式测试了十周。骗子组为基础饮食，VE组，KAE组和KAE + VE组喂食基础饮食，将其加入0.2 g/kg VE，0.4 g/kg Kae和0.2 g/kg VE + +分别为0.4 g/kg kae。结果如下。与CON组相比，VE组，KAE组和KAE + VE组显着提高了鸡蛋的产量，平均每日鸡蛋体重并显着降低了饲料与蛋的比率。VE + KAE组显着改善了豪gh单元。VE组，KAE组和KAE + VE组大大提高了蛋壳强度，蛋壳相对重量，蛋壳厚度，蛋黄颜色和相对蛋黄的重量。KAE组和KAE + VE组的血清E2和LH水平以及KAE + VE组的血清FSH水平明显更高。在卵巢中，KAE组和KAE + VE组的ESR1基因表达水平明显更高，并且KAE + VE组的FSHR基因表达水平明显更高。在子宫中，KAE组和KAE + VE组的ESR1基因表达水平较高，KAE + VE组的ESR2和FSHR基因表达水平明显更高。16S rRNA基因测序表明，β多样性PCOA中盲肠和子宫菌落的显着聚集。在盲肠中，富公司，杀菌剂和WPS-2是主要的门。在子宫中，坚硬，蛋白质细菌和杆菌是主要的门。KAE + VE组的F/B在门水平上明显高于CON组和VE组。总而言之，将VE和KAE添加到饮食中可以改善晚期母鸡的产生性能，增加生殖激素的含量，并稳定盲肠和子宫菌群，这可能与激素和微生物群有关肠道轴。