摘要
ABSTRACT
Differences in rumen volatile fatty acids (VFAs), microbial population and community composition between water buffalo and Jersey cows were compared to explore the effects of animal species on ruminal microbiota. Ruminally fistulated buffalo (n = 4) and Jersey cows (n = 4) with similar body weights were selected and provided with similar diets. After feeding for 30 days, rumen fluid was collected to quantify the ruminal VFA, and determine microbial population and community composition. Results revealed that concentrations of total VFA, acetate and propionate in buffalo were higher (P > .05), while butyrate concentration and acetate/propionate ratio were lower than those in Jersey cows (P < .05). Populations of ruminal bacteria, protozoa, fungi and F. succinogenes were higher in buffalo (P < .05). The abundance of Firmicutes was higher (P < .05), while the abundance of Bacteroidetes was lower in buffalo (P < .05). The abundances of dominant bacterial genus Prevotella and dominant methanogen genus Methanobrevibacter were higher in Jersey cows (P < .05). Protozoal genus Metadinium was dominant in buffalo, while Entodinium was dominant in Jersey cows. Concluded that not only rumen microbial population, but also microbial community composition were different between two species. The higher bacterial, protozoal and fungal populations, and different microbial community can explain why buffalo are more efficient to digest fibre.
摘要译文
摘要比较了水牛和泽西奶牛之间瘤胃挥发性脂肪酸（VFA），微生物种群和群落组成的差异，探讨了动物种对瘤胃微生物群的影响。选择具有相似体重的瘤胃瘘水牛（n = 4）和泽西奶牛（n = 4）并提供相似的饮食。饲喂30天后，收集瘤胃液以量化瘤胃VFA，并确定微生物种群和群落组成。结果显示，水牛中总VFA，乙酸盐和丙酸盐的浓度较高（P> .05），而丁酸盐浓度和醋酸盐/丙酸盐比率低于泽西奶牛（P <.05）。瘤胃细菌，原生动物，真菌和F. succinogenes的种群在水牛中较高（P <.05）。厚壁菌的丰度较高（P <.05），而水牛的拟杆菌丰度较低（P <.05）。优势细菌属Prevotella和显性产甲烷菌属Methanobrevibacter的丰度在泽西奶牛中较高（P <.05）。原生动物Metadinium在水牛中占优势，而Entodinium在泽西奶牛中占优势。得出结论，不仅瘤胃微生物种群，而且两种植物的微生物群落组成也不同。较高的细菌，原生动物和真菌种群以及不同的微生物群落可以解释为什么水牛更有效地消化纤维。
Muhammad Wasim Iqbal[1];Qin Zhang[1];Yingbai Yang[1];Lili Li[2];Caixia Zou[1];Chunhua Huang[3];Bo Lin[4]. Comparative study of rumen fermentation and microbial community differences between water buffalo and Jersey cows under similar feeding conditions[J]. Journal of Applied Animal Research, 2018,46(1): 740-748