期刊文献

Seed Priming with 2,4-Epibrassionolide Enhances Seed Germination and Heat Tolerance in Rice by Regulating the Antioxidant System and Plant Hormone Signaling Pathways 收藏

用2,4-纤维固醇启动种子启动通过调节抗氧化剂系统和植物激素信号通路，从而增强种子的发芽和耐热性

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作者

Jingya Qian [1];Xu Mo [2];Yue Wang [3];Qiang Li [4];

作者单位

[1]College of Agronomy, Hunan Agricultural University, Changsha 410128, China; [2]The Key Laboratory of Crop Germplasm Innovation and Resource Utilization of Hunan Province, Hunan Agricultural University, Changsha 410128, China; [3]Yuelu Mountain Laboratory of Hunan Province, Hunan Agricultural University, Changsha 410128, China; [4]College of Agronomy, Hunan Agricultural University, Changsha 410128, China;

发布日期

2025-2-19

页码

242

DOI

10.3390/antiox14020242

来源信息

Antioxidants ISSN：2076-3921, 2025年, 14卷, 2期, 242页

摘要

With global climate warming, enhancing the heat stress tolerance of rice seeds is critical for ensuring crop yields and maintaining global food security. 2,4-Epibrassionolide (EBR) has been shown to effectively alleviate the adverse effects of heat stress on rice seed germination, but its mitigation mechanism has not been fully clarified. In this experiment, exogenous EBR was used as a seed priming agent. The activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), malondialdehyde (MDA), soluble protein contents, and plant hormone levels were measured during rice seed germination under heat stress (38 °C). We constructed a cDNA library for transcriptome sequencing analysis. The results showed that exogenous EBR could effectively alleviate the effect of heat stress on rice seeds by enhancing SOD, POD, and CAT enzyme activity; reducing the MDA content; and increasing the soluble protein content. Additionally, exogenous EBR increases the levels of GA and IAA while decreasing the ABA content. According to a transcriptomic analysis, exogenous EBR can induce the expression of key genes involved in GA, IAA, and ABA hormone biosynthesis and metabolism, regulating GA-, IAA-, ABA-, and H2O2-mediated signaling pathways to promote the germination of rice seeds under heat stress. This study provides new insights into the application of rice seed priming techniques.

摘要译文

随着全球气候变暖，增强水稻种子的热应激耐受性对于确保作物产量和维持全球粮食安全至关重要。已显示2,4-纤维氨基酚（EBR）有效缓解热应激对水稻种子发芽的不利影响，但尚未完全阐明其缓解机制。在该实验中，外源EBR用作种子启动剂。在热应激下（38°C）下，测量了稻种子籽发芽期间测量了超氧化物歧化酶（SOD），过氧化物酶（POD），过氧化氢酶（CAT），丙二醛（MDA），可溶性蛋白质含量和植物激素水平的活性。我们构建了一个用于转录组测序分析的cDNA库。结果表明，外源EBR可以通过增强SOD，POD和CAT酶活性有效地减轻热应激对水稻种子的影响。减少MDA含量；并增加可溶性蛋白质含量。此外，外源EBR增加了GA和IAA的水平，同时降低了ABA含量。根据转录组分析，外源EBR可以诱导参与GA，IAA和ABA激素生物合成和代谢的关键基因的表达，调节GA-，IAA-，ABA-和H2O2介导的介导的信号通路，以在热应激下促进水稻种子的发芽。这项研究为水稻种子启动技术的应用提供了新的见解。

Jingya Qian [1];Xu Mo [2];Yue Wang [3];Qiang Li [4];. Seed Priming with 2,4-Epibrassionolide Enhances Seed Germination and Heat Tolerance in Rice by Regulating the Antioxidant System and Plant Hormone Signaling Pathways[J]. Antioxidants, 2025,14(2): 242