摘要
A liquid chromatographic method for determining residues of ionophores in bovine, porcine, and avian tissues is described. Tissues were extracted with iso‐octane‐ethyl acetate and the extracts purified on silica solid‐phase extraction columns. Monensin, narasin and salinomycin were detected by UV absorbance following post‐column derivatization with vanillin, and lasalocid was detected underivatized using fluorescence. In muscle, kidney and fat, all drugs were determined from a single sample extract. In liver, a separate extraction was done to determine lasalocid. The detection limit for lasalocid, narasin and salinomycin was 5 ppb; for monensin it was 2ppb. Average recoveries were: lasalocid—71.0%; monensin—94.3%; salinomycin—97.2%; narasin—94.1 %.
摘要译文
描述了用于测定牛，猪和禽组织中的离子载体残留的液相色谱方法。用异辛烷 - 乙酸乙酯萃取组织，并在二氧化硅固相萃取柱上纯化萃取物。在用香草醛进行柱后衍生后，通过UV吸光度检测莫能菌素，narasin和盐霉素，并且使用荧光检测拉沙里菌素未衍生化。在肌肉，肾脏和脂肪中，所有药物都是从单一样品提取物中确定的。在肝脏中，进行单独的提取以确定拉沙里菌素。拉沙里菌素，narasin和沙利霉素的检出限为5 ppb;对于莫能菌素，它是2ppb。平均回收率为：拉沙里菌素-71.0％;莫能菌素-94.3％;沙利霉素-97.2％; narasin-94.1％。
Geoff C. Gerhardt [1];Craig D. C. Salisbury [1];Harold M. Campbell [2]. Determination of ionophores in the tissues of food animals by liquid chromatography[J]. Food Additives & Contaminants, 1995,12(6): 731-737