期刊文献

Correlated Responses to Selection for Intramuscular Fat on the Gut Microbiome in Rabbits 收藏

兔子肠内微生物组的肌内脂肪选择的响应

原文求助发布源

作者

Marina Martínez-Álvaro (0000-0003-2295-5839) [1];Agostina Zubiri-Gaitán (0000-0001-9254-2461) [2];Pilar Hernández (0000-0001-7857-7334) [3];Cristina Casto-Rebollo [4];Noelia Ibáñez-Escriche (0000-0002-6221-3576) [5];Maria Antonia Santacreu [6];Alejandro Artacho [7];Vicente Pérez-Brocal [8];Agustín Blasco (0000-0003-3558-0810) [9];

作者单位

[1]Institute for Animal Science and Technology, Universitat Politècnica de València, 46022 Valencia, Spain; [2]Institute for Animal Science and Technology, Universitat Politècnica de València, 46022 Valencia, Spain; [3]Institute for Animal Science and Technology, Universitat Politècnica de València, 46022 Valencia, Spain; [4]Institute for Animal Science and Technology, Universitat Politècnica de València, 46022 Valencia, Spain; [5]Institute for Animal Science and Technology, Universitat Politècnica de València, 46022 Valencia, Spain; [6]Institute for Animal Science and Technology, Universitat Politècnica de València, 46022 Valencia, Spain; [7]Area of Genomics and Health, Foundation for the Promotion of Sanitary and Biomedical Research of Valencia Region (FISABIO-Public Health), 46022 Valencia, Spain; [8]Area of Genomics and Health, Foundation for the Promotion of Sanitary and Biomedical Research of Valencia Region (FISABIO-Public Health), 46022 Valencia, Spain; [9]Institute for Animal Science and Technology, Universitat Politècnica de València, 46022 Valencia, Spain;

发布日期

2024-7-16

页码

2078

DOI

10.3390/ani14142078

来源信息

Animals ISSN：2076-2615, 2024年, 14卷, 14期, 2078页

摘要

Intramuscular fat (IMF) content is important for meat production and human health, where the host genetics and its microbiome greatly contribute to its variation. The aim of this study is to describe the consequences of the genetic modification of IMF by selecting the taxonomic composition of the microbiome, using rabbits from the 10th generation of a divergent selection experiment for IMF (high (H) and low (L) lines differ by 3.8 standard deviations). The selection altered the composition of the gut microbiota. Correlated responses were better distinguished at the genus level (51 genera) than at the phylum level (10 phyla). The H-line was enriched in Hungateiclostridium, Limosilactobacillus, Legionella, Lysinibacillus, Phorphyromonas, Methanosphaera, Desulfovibrio, and Akkermansia, while the L-line was enriched in Escherichia, Methanobrevibacter, Fonticella, Candidatus Amulumruptor, Methanobrevibacter, Exiguobacterium, Flintibacter, and Coprococcus, among other genera with smaller line differences. A microbial biomarker generated from the abundance of four of these genera classified the lines with 78% accuracy in a logit regression. Our results demonstrate different gut microbiome compositions in hosts with divergent IMF genotypes. Furthermore, we provide a microbial biomarker to be used as an indicator of hosts genetically predisposed to accumulate muscle lipids, which opens up the opportunity for research to develop probiotics or microbiome-based breeding strategies targeting IMF.

摘要译文

肌内脂肪（IMF）含量对于肉类生产和人类健康很重要，在这种情况下，宿主遗传学及其微生物组极大地有助于其变异。这项研究的目的是使用第十代IMF的第十代选择实验中的兔子（高（H）和低（H）和低（L）线不同的兔子来描述IMF遗传修饰的后果。由3.8标准偏差）。选择改变了肠道菌群的组成。在属水平（51属）上，相关反应比在门水平（10个门）上更好地区分。H-线在HungateClostridium，Limosilactobacillus，军团菌，脂肪菌，phorphyomonas，甲磷酸，Desulfovibrio和Akkermansia中，而L线在Escherichia，Escherichia，甲基甲基甲基菌，Fontiber，fintusell，criber and trancus and trandus and tranciber，trangus and trancus and tranciber，，弗林特杆菌和cocrococcus，除其他属差异较小的属外。从这些属的四个属中产生的微生物生物标志物在logit回归中以78％的精度分类。我们的结果表明，具有不同IMF基因型的宿主中不同的肠道微生物组组成。此外，我们提供了一种微生物生物标志物，可用作遗传上易于积累肌肉脂质的宿主的指标，这为研究旨在开发益生菌或基于微生物组的靶向IMF的育种策略提供了机会。