摘要
This article presents a new method of using molecular markers to study the material base of the herbal nature of traditional Chinese medicine (TCM) herbs. The feasibility of using the all-electric ion chromatography to select the appropriate protein molecular markers for studying the herbal nature of TCM herbs is also discussed. In the study, the chromatographic peaks of the total water-soluble proteins of a TCM herb in a specific pH value condition were selected using anion-exchange chromatography in combination with cation exchange chromatography. This method is known as the all-electric ion chromatography. The common chromatographic peaks of TCM herbs with same herbal nature were sorted out by parallel comparison of different all-electric ion chromatograms. The all-electric ion chromatograms could be used to mark the herbal nature of TCM herbs. SDS-PAGE was performed on the common chromatographic peak fractions. The common electrophoresis bands of TCM herbs with same herbal nature were selected as the molecular markers for the corresponding herbal nature. Test results showed that near the fraction number of 30 in anion-ion exchange chromatography, there were several common chromatographic peaks for three tested TCM herbs with hot nature: Rhizoma zingiberis, Cortex cinnamomi, and Rhizoma curculignis. These three TCM have three common protein electrophoresis bands at the molecular weight of 51 kU on 12% SDS-PAGE gel. As for three TCM herbs with cold nature: Rhizoma coptidis, Rhizoma anemarrhenae, and Flos lonicerea japonicae, the common chromatographic peaks were found near the fraction number of 35 in anion-ion exchange chromatography. These cold nature TCM herbs have two common electrophoresis bands at the molecular weight of near 31 kU on 12% SDS-PAGE gel. Common chromatographic peaks of TCM herbs studied in this research showed three repeats in all-electric ion chromatograms. These common chromatographic peaks could be used to mark the corresponding herbal nature of TCM herbs. Common protein electrophoresis bands of the common chromatographic peak fractions were specific for three hot TCM herbs and three cold TCM herbs studied in this research. Hence, they can be used as the protein molecular markers for studying the herbal nature of all these six TCM herbs. All-electric ion chromatography is a new desirable method for screening molecular markers of the herbal nature of TCM herbs.
摘要译文
本文介绍了一种利用分子标记研究中药草药性质的物质基础的新方法。还讨论了使用全电离子色谱法选择合适的蛋白质分子标记来研究中草药草药性质的可行性。在该研究中，使用阴离子交换色谱法结合阳离子交换色谱法选择特定pH值条件下的中草药总水溶性蛋白质的色谱峰。该方法称为全电离子色谱法。通过不同全电离子色谱图的平行比较，对具有相同草药性质的中草药的常见色谱峰进行了分类。全电离子色谱图可用于标记中草药的草药性质。对常见的色谱峰级分进行SDS-PAGE。选择具有相同草药性质的中草药的常用电泳条带作为相应草药性质的分子标记。试验结果表明，在阴离子交换色谱中接近30的分数，对于三种具有热性质的中药材，有几种常见的色谱峰：莪术，肉桂和枸杞（Rhizoma curculignis）。这三种TCM在12％SDS-PAGE凝胶上具有三个分子量为51kU的常见蛋白质电泳条带。对于三种具有冷性的中草药：黄连，天竺葵和日本金银花，在阴离子交换色谱中，在35的分数附近发现了常见的色谱峰。这些冷天然中草药在12％SDS-PAGE凝胶上具有两个常见的电泳条带，分子量接近31 kU。本研究中研究的中草药常见色谱峰在全电离子色谱图中显示三次重复。这些常见的色谱峰可用于标记中草药的相应草药性质。常见的色谱峰级分的常见蛋白质电泳条带对于本研究中研究的三种热TCM草药和三种冷TCM草药具有特异性。因此，它们可以作为蛋白质分子标记用于研究所有这六种中草药的草药性质。全电离子色谱法是筛选中草药草药性质分子标记的新方法。
WangHou-wei;DouYan-ling;TianJing-zhen;WangZhen-guo;. Protein Molecular Markers for Herbal Natures of Six Traditional Chinese Medicinal Herbs[J]. World Science and Technology, 2008,10(6): 27-31