摘要
The domestic ferret (Mustela putorius furo) is an important model organism for the study of avian influenza and other diseases of humans and animals, as well as a popular pet animal. In order to evaluate genetic diversity and study disease relationships in ferrets, 22 nuclear microsatellite loci (17 dinucleotide and 5 tetranucleotide) were developed from ferret genomic libraries and organized into seven multiplex sets. Polymorphism was preliminarily assessed in one population in Australia and one in the USA, sampled with 25 individuals each. The loci displayed allelic diversity ranging from 1 to 5 alleles, and expected and observed heterozygosities ranging from 0.04 to 0.65 and 0.04 to 0.76, respectively. Additionally, the loci amplified products in 15 samples from the wild ancestor, European polecat (Mustela putorius) and domestic ferret-polecat hybrids. In polecat/hybrid samples, allelic diversity ranged from 3 to 8 alleles, and expected and observed heterozygosities ranged from 0.13 to 0.81 and 0.13 to 0.80 respectively. These markers will be useful for molecular assessments of genetic diversity and applications to evolution, ecology, and health in domestic ferrets and wild polecats.
摘要译文
家鼬（Mustela putorius furo）是研究禽流感和其他人类和动物疾病以及受欢迎的宠物动物的重要模式生物。为了评估雪貂的遗传多样性和研究疾病关系，从雪貂基因组文库中开发出22个核微卫星位点（17个二核苷酸和5个四核苷酸），并组织成7个多重组。在澳大利亚的一个种群和美国的一个种群中初步评估了多态性，每个种群有25个个体。该基因座显示1至5个等位基因的等位基因多样性，预期和观察到的杂合度分别为0.04至0.65和0.04至0.76。此外，该基因座扩增了来自野生祖先，欧洲臭鼬（Mustela putorius）和家鼬 - 臭鼬杂交种的15个样品中的产物。在臭鼬/杂交样品中，等位基因多样性范围为3至8个等位基因，预期和观察到的杂合度分别为0.13至0.81和0.13至0.80。这些标记可用于遗传多样性的分子评估以及家养雪貂和野生鸡貂的进化，生态学和健康的应用。
Holly B. Ernest[ 1;2;][*] ; Tracy L. Drazenovich[ 3]; Lisa S. Dalbeck[ 1];Michelle G. Hawkins[ 3]. Isolation and Characterization of Microsatellite Markers in the Domestic Ferret (Mustela putorius furo)[J]. International Journal of Molecular Sciences, 2012,13(12): 16592-16597