期刊文献

Transcriptomic Comparison of Rice lncRNAs in Response to Feeding by Brown Planthopper Populations with Different Virulence 收藏

水稻lncrNA的转录组比较，响应于棕色的planthopper种群的喂养，具有不同的毒力
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作者
Yaxuan Wang [1];Xinfeng Wang [2];Kunjie Zhang [3];Jing Xiao [4];Fang Liu [5];Houhong Yang [6];Yubiao Cai [7];Fengxiang Lai [8];Qiang Fu [9];Pinjun Wan (https://orcid.org/0000-0002-6786-2567) [10];
作者单位
[1]The National Key Laboratory of Rice Biological Breeding, China National Rice Research Institute, Hangzhou 311401, China; [2]The National Key Laboratory of Rice Biological Breeding, China National Rice Research Institute, Hangzhou 311401, China; [3]The National Key Laboratory of Rice Biological Breeding, China National Rice Research Institute, Hangzhou 311401, China; [4]The National Key Laboratory of Rice Biological Breeding, China National Rice Research Institute, Hangzhou 311401, China; [5]The National Key Laboratory of Rice Biological Breeding, China National Rice Research Institute, Hangzhou 311401, China; [6]The National Key Laboratory of Rice Biological Breeding, China National Rice Research Institute, Hangzhou 311401, China; [7]The National Key Laboratory of Rice Biological Breeding, China National Rice Research Institute, Hangzhou 311401, China; [8]The National Key Laboratory of Rice Biological Breeding, China National Rice Research Institute, Hangzhou 311401, China; [9]The National Key Laboratory of Rice Biological Breeding, China National Rice Research Institute, Hangzhou 311401, China; [10]The National Key Laboratory of Rice Biological Breeding, China National Rice Research Institute, Hangzhou 311401, China;
发布日期
2025-4-8
页码
3486
DOI
10.3390/ijms26083486
来源信息
International Journal of Molecular Sciences ISSN：1422-0067, 2025年, 26卷, 8期, 3486页
摘要
The brown planthopper (BPH) is one of the major rice pests causing significant damage to rice production worldwide, due to its high reproductive capacity and strong migratory ability. A series of BPH-resistant rice varieties have been developed, but the durability of effective resistance is constrained by the evolution of BPH virulence, requiring in-depth insights into resistance mechanisms. In this paper, we used strand-specific lncRNA-seq to characterize the lncRNA regulatory mechanisms on rice response to BPH infestation. Overall, 4321 lncRNAs were identified, 60 of which were significantly upregulated in response to BPH infestation, specifically differing between BPH populations with variable virulence. Differential expression analysis and qRT-PCR validation showed that these lncRNAs are involved in the regulation of several defense pathways, including jasmonic acid signaling and flavonoid biosynthesis, with their distinct roles in resistant and susceptible rice varieties. Notably, lncRNAs like LNC_002533 were found to be negatively correlated with flavonoid biosynthesis, suggesting a potential role in modulating rice defense responses. In contrast, LNC_001986 and LNC_000397 were positively correlated with genes involved in glutathione metabolism, which may be associated with enhanced resistance. These findings highlight the critical regulatory functions of lncRNAs in rice-BPH interactions and provide a molecular framework for improving rice resistance through targeted genetic engineering. This study significantly contributes to functional genomics by elucidating lncRNA-mediated regulatory mechanisms and offers promising avenues for developing durable pest-resistant rice varieties.
摘要译文
棕色的Planthopper（BPH）是主要的大米害虫之一，由于其高生殖能力和强大的迁徙能力，在全球范围内造成了重大损害。已经开发了一系列耐BPH的水稻品种，但是有效抗性的耐用性受到BPH毒力的演变的限制，需要深入了解耐药机制。在本文中，我们使用链特异性的lncRNA-seq来表征lncRNA调节机制，这些机制在水稻对BPH感染的反应上。总体而言，确定了4321个LNCRNA，其中60个因响应BPH的侵染而显着上调，这在具有可变毒力的BPH种群之间有所不同。差异表达分析和QRT-PCR验证表明，这些LNCRNA参与了几种防御途径的调节，包括茉莉酸信号传导和类黄酮生物合成，在耐药性和易感水稻品种中具有独特的作用。值得注意的是，发现像LNC_002533这样的LNCRNA与类黄酮生物合成呈负相关，这表明在调节水稻防御反应中具有潜在的作用。相比之下，LNC_001986和LNC_000397与参与谷胱甘肽代谢的基因呈正相关，这可能与增强的耐药性有关。这些发现突出了LNCRNA在水稻相互作用中的关键调节功能，并提供了通过靶向基因工程改善水稻耐药性的分子框架。这项研究通过阐明LNCRNA介导的调节机制来显着有助于功能基因组学，并为开发耐用的害虫含量的水稻品种提供了有希望的途径。
Yaxuan Wang [1];Xinfeng Wang [2];Kunjie Zhang [3];Jing Xiao [4];Fang Liu [5];Houhong Yang [6];Yubiao Cai [7];Fengxiang Lai [8];Qiang Fu [9];Pinjun Wan (https://orcid.org/0000-0002-6786-2567) [10];. Transcriptomic Comparison of Rice lncRNAs in Response to Feeding by Brown Planthopper Populations with Different Virulence[J]. International Journal of Molecular Sciences, 2025,26(8): 3486