摘要
Macrophages are among the main cells involved in generation of inflammation. To investigate the anti-inflammatory effect of Satureja hortensis (summer savory), lipopolysaccharide (LPS)-activated J774.1 macrophages were treated with various extracts, and the expression and release of various inflammatory molecules by macrophages were examined. We showed that dichloromethane and hexane extracts reduced nitric oxide (NO) production more efficiently than other extracts. Both extracts decreased gene expression of inducible NO synthase (iNOS) (<0.44 fold of control), cyclooxygenase (COX)-2 (<0.29 fold), interleukin (IL)-1β (<0.41 fold), IL-6 (<0.25 fold) and tumor necrosis factor (TNF)-α (<0.2 fold). The extracts reduced IL-6 and IL-1β proteins production from macrophages. Surface intensity of expression of intercellular adhesion molecule (ICAM)-1 decreased to 845 ± 28.1 (dichloromethane) and 715 ± 48.6 (hexane) compared to the control (902 ± 73.1). These findings showed that Satureja hortensis, by influencing macrophages and related mediators, could contribute to reduction of inflammation and might be useful as an anti-inflammatory agent.
摘要译文
巨噬细胞是参与炎症产生的主要细胞之一。为了研究Satureja hortensis（夏季咸味）的抗炎作用，用各种提取物处理脂多糖（LPS）激活的J774.1巨噬细胞，并检查巨噬细胞表达和释放各种炎性分子。我们发现二氯甲烷和己烷提取物比其他提取物更有效地减少了一氧化氮（NO）的产生。两种提取物均降低诱导型一氧化氮合酶（iNOS）（对照的0.44倍），环氧合酶（COX）-2（<0.29倍），白细胞介素（IL）-1β（<0.41倍），IL-6（<0.25）的基因表达。折叠）和肿瘤坏死因子（TNF）-α（<0.2倍）。提取物减少了巨噬细胞产生的IL-6和IL-1β蛋白质。与对照（902±73.1）相比，细胞间粘附分子（ICAM）-1的表达强度降低至845±28.1（二氯甲烷）和715±48.6（己烷）。这些发现表明，通过影响巨噬细胞和相关介质，Satureja hortensis可以有助于减少炎症，并且可以用作抗炎剂。
Zahra Farzaneh[1];Kurosh Kalantar[1];Aida Iraji[2];Zahra Amirghofran[3]. Inhibition of LPS-induced inflammatory responses by Satureja hortensis extracts in J774.1 macrophages[J]. Journal of Immunoassay, 2018,39(3): 274-291