期刊文献

Unlocking Fertility: How Nitric Oxide Pathways Connect Obesity and Reproductive Health—The Role of Bariatric Surgery 收藏

解锁生育能力：一氧化氮途径如何连接肥胖和生殖健康 - 减肥手术的作用

原文求助发布源

作者

Charalampos Voros (https://orcid.org/0000-0001-9477-7619) [1];Despoina Mavrogianni (https://orcid.org/0000-0003-1220-6594) [2];Kyriakos Bananis [3];Antonia Varthaliti (https://orcid.org/0009-0008-8725-7103) [4];Anthi-Maria Papahliou [5];Vasileios Topalis [6];Panagiota Kondili [7];Menelaos Darlas [8];Maria Anastasia Daskalaki [9];Agni Pantou (https://orcid.org/0000-0003-3224-5646) [10];Diamantis Athanasiou [11];Dimitris Mathiopoulos [12];Marianna Theodora (https://orcid.org/0000-0001-8280-7796) [13];Panagiotis Antsaklis (https://orcid.org/0000-0003-2106-5922) [14];Dimitrios Loutradis (https://orcid.org/0000-0002-8686-594X) [15];Georgios Daskalakis (https://orcid.org/0000-0001-7108-211X) [16];

作者单位

[1]1st Department of Obstetrics and Gynecology, ‘Alexandra’ General Hospital, National and Kapodistrian University of Athens, 80 Vasilissis Sofias Avenue, 11528 Athens, Greece; [2]1st Department of Obstetrics and Gynecology, ‘Alexandra’ General Hospital, National and Kapodistrian University of Athens, 80 Vasilissis Sofias Avenue, 11528 Athens, Greece; [3]King’s College Hospitals NHS Foundation Trust, London SE5 9RS, UK; [4]1st Department of Obstetrics and Gynecology, ‘Alexandra’ General Hospital, National and Kapodistrian University of Athens, 80 Vasilissis Sofias Avenue, 11528 Athens, Greece; [5]1st Department of Obstetrics and Gynecology, ‘Alexandra’ General Hospital, National and Kapodistrian University of Athens, 80 Vasilissis Sofias Avenue, 11528 Athens, Greece; [6]Department of Internal Medicine, Hospital of Thun, 3600 Thun, Switzerland; [7]1st Department of Obstetrics and Gynecology, ‘Alexandra’ General Hospital, National and Kapodistrian University of Athens, 80 Vasilissis Sofias Avenue, 11528 Athens, Greece; [8]1st Department of Obstetrics and Gynecology, ‘Alexandra’ General Hospital, National and Kapodistrian University of Athens, 80 Vasilissis Sofias Avenue, 11528 Athens, Greece; [9]1st Department of Obstetrics and Gynecology, ‘Alexandra’ General Hospital, National and Kapodistrian University of Athens, 80 Vasilissis Sofias Avenue, 11528 Athens, Greece; [10]1st Department of Obstetrics and Gynecology, ‘Alexandra’ General Hospital, National and Kapodistrian University of Athens, 80 Vasilissis Sofias Avenue, 11528 Athens, Greece; [11]IVF Athens Reproduction Center V. Athanasiou, 151 23 Maroussi, Greece; [12]Rea Maternity Hospital S.A., Avenue Siggrou 383 &Pentelis 17, P. Faliro, 17564 Athens, Greece; [13]1st Department of Obstetrics and Gynecology, ‘Alexandra’ General Hospital, National and Kapodistrian University of Athens, 80 Vasilissis Sofias Avenue, 11528 Athens, Greece; [14]1st Department of Obstetrics and Gynecology, ‘Alexandra’ General Hospital, National and Kapodistrian University of Athens, 80 Vasilissis Sofias Avenue, 11528 Athens, Greece; [15]Fertility Institute-Assisted Reproduction Unit, Paster 15, 11528 Athens, Greece; [16]1st Department of Obstetrics and Gynecology, ‘Alexandra’ General Hospital, National and Kapodistrian University of Athens, 80 Vasilissis Sofias Avenue, 11528 Athens, Greece;

发布日期

2025-2-19

页码

240

DOI

10.3390/antiox14020240

来源信息

Antioxidants ISSN：2076-3921, 2025年, 14卷, 2期, 240页

摘要

This study examines the relationship between obesity, oxidative stress, and reproductive dysfunction. It focuses on the effects of sleeve gastrectomy on gene expression and hormone profiles in 29 women with severe obesity (BMI ≥ 40 kg/m2). Pre- and post-surgical investigations revealed significant differences in major gene expressions and hormonal markers. CART expression reduced significantly from 0.27 ± 4.43 to −3.42 ± 1.14 (p < 0.001), while leptin expression decreased from −1.87 ± 1.75 to −0.13 ± 1.55 (p < 0.001), indicating better metabolic regulation. In contrast, eNOS expression increased considerably from −4.87 ± 1.70 to 1.18 ± 2.31 (p = 0.003), indicating improved endothelial function and nitric oxide bioavailability, which is critical for vascular health and reproduction. Correlation research before surgery indicated no significant relationships between eNOS, CART, or leptin and clinical indicators, implying that these genes function independently in pre-surgical metabolism. While most associations remained negligible after surgery, a significant negative connection between eNOS expression and SHBG levels appeared (r = −0.365, p = 0.049), indicating potential interactions in hormonal regulation pathways following metabolic improvements. These findings emphasize the importance of bariatric surgery in reducing the negative effects of obesity on reproductive health by altering critical cellular pathways. Significant increases in CART, leptin, and eNOS expression indicate reduced oxidative stress, improved vascular tone, and hormonal balance, all of which contribute to increased reproductive capacity. This study sheds light on the molecular processes that link obesity, metabolic health, and fertility, underlining bariatric surgery’s therapeutic potential for women experiencing obesity-related infertility.

摘要译文

这项研究检查了肥胖，氧化应激和生殖功能障碍之间的关系。它着重于袖胃切除术对29名严重肥胖女性（BMI≥40kg/m2）的基因表达和激素谱的影响。手术前和手术后的研究表明，主要基因表达和荷尔蒙标记的显着差异。CART表达从0.27±4.43显着降低至-3.42±1.14（p <0.001），而瘦素表达从-1.87±1.75降至-0.13±1.55（p <0.001），表明更好地代谢。相反，eNOS的表达从-4.87±1.70升至1.18±2.31（p = 0.003），表明内皮功能的改善和一氧化氮生物利用度，这对于血管健康和生殖至关重要。手术前的相关研究表明，eNOS，CART或LEPTIN和临床指标之间没有显着关系，这表明这些基因在手术前代谢中独立起作用。虽然大多数关联在手术后仍然可以忽略不计，但出现eNOS表达与SHBG水平之间的显着负相关（r = -0.365，p = 0.049），表明在代谢改进后，激素调节途径中的潜在相互作用。这些发现强调了减肥手术对通过改变关键细胞途径来减少肥胖对生殖健康的负面影响的重要性。CART，瘦素和eNOS表达的显着增加表明氧化应激降低，血管张力的改善和激素平衡，所有这些都有助于提高生殖能力。这项研究阐明了将肥胖，代谢健康和生育能力联系起来的分子过程，这突显了减肥手术对与肥胖相关不育症的女性的治疗潜力。