摘要
During the breeding seasons of 1989 and 1990, a total of 617 blue fox vixens aged 1 to 6 years (mean ± SEM, 2.6 ± 0.1) were inseminated with frozen silver fox semen with either 150 million (n = 213, 1989 + 1990), 100 million (n=172, 1990), 75 million (n = 119, 1989) or 37.5 million (n = 113, 1989) spermatozoa per insemination. Two intrauterine inseminations, each with an insemination volume of 1.0 ml, were performed at 24-hour intervals on the first and second days after maximum vaginal electrical resistance was measured.
Conception rates were 87% (186 of 213) with 150 million spermatozoa per insemination, 85% (146 of 172) with 100 million, 77% (91 of 119) with 75 million and 68% (77 of 113) with 37.5 million. The mean numbers of cubs per litter ± SEM for the four groups were 7.6 ± 0.2 (168 registered litters), 7.5 ± 0.3 (115 litters), 6.4 ± 0.4 (86 litters) and 6.4 ± 0.4 (75 litters). A negative effect on both the conception rate and mean litter size at whelping was observed with decreasing sperm numbers (conception rate percentage: p = 0.0001, Chi-square, litter size: p = 0.02, Kruskal-Wallis Test). Only the two larger numbers of spermatozoa gave litter sizes comparable to those obtained by artificial insemination (AI) with fresh semen.
摘要译文
在1989年和1990年的繁殖季节，共有617只年龄为1至6岁的蓝狐（平均值±SEM，2.6±0.1）用冷冻银狐精液进行授精，其中1.5亿（n = 213,1989 + 1990）每次授精1亿（n = 172,1990），7500万（n = 119,1989）或3750万（n = 113,1989）精子。在测量最大阴道电阻后的第一天和第二天，以24小时间隔进行两次子宫内授精，每次授精体积为1.0ml。受孕率为87％（213人中的186人），每次授精1.5亿精子，85％（172人中的146人），1亿人，77％（119人中的91人），7500万人和68％（113人中的77人），3750万人。四组的平均每窝幼仔数±SEM为7.6±0.2（168个登记的窝），7.5±0.3（115窝），6.4±0.4（86窝）和6.4±0.4（75窝）。随着精子数量的减少，观察到对幼仔的受胎率和平均产仔数的负面影响（受孕率百分比：p = 0.0001，卡方，产仔数：p = 0.02，Kruskal-Wallis检验）。只有两个较大数量的精子产生的垫料大小与通过人工授精（AI）与新鲜精液获得的相似。
W.Farstad[1][a];J.A.Fougner[2];C.G.Torres[3];. The effect of sperm number on fertility in blue fox vixens (Alopex[J]. Theriogenology, 1992,37(3): 699-711