摘要
Hemicelluloses, the second most abundant polysaccharides right after cellulose, represent complex biopolymers whose structural features are not yet fully understood. Hence, the applicability of functionalized hemicellulose derivatives is still limited. Thus, the development of practical methods to reveal their structure is essential. Anionic, carboxylated GGM was prepared as the substrate, and their corresponding oligomers have been isolated and fully characterized for the first time in the present work. In order to accomplish this, each step has been optimized sequentially. Oxidized GGM was enzymatically digested, and the corresponding oligomers were isolated by preparative GPC. A toolset of wet chemistry and instrumental methods was used to characterize the isolated compounds in detail. Moreover, quantitative 13C-NMR combined with MALDI was demonstrated to deliver accurate analytical data compared to standard but time-consuming wet chemistry methods. The developed toolset is expected to be applicable to other plant polysaccharides and their derivatives as well.
摘要译文
半纤维素是纤维素之后第二丰富的多糖，代表复杂的生物聚合物，其结构特征尚未完全了解。因此，官能化半纤维素衍生物的适用性仍然是有限的。因此，开发显示其结构的实用方法是必不可少的。制备阴离子羧化GGM作为底物，并且在本工作中首次分离并充分表征了它们相应的低聚物。为了实现这一点，每个步骤都已按顺序进行优化。酶促消化氧化的GGM，并通过制备型GPC分离相应的寡聚物。使用湿化学和仪器方法的工具集来详细表征分离的化合物。此外，与标准但耗时的湿化学方法相比，与MALDI结合的定量 13 C-NMR被证明可提供准确的分析数据。预计开发的工具集也适用于其他植物多糖及其衍生物。
T. Eichinger[1]]J. Rahkila[2]]S. Willför[1]]C. Xu[1]]. TEMPO-oxidized O-acetyl galactoglucomannan oligomers: isolation and comprehensive structural elucidation[J]. Wood Science and Technology, 2019,53(1): 71-85