摘要
Study was conducted to compare the semen quality and fertility of liquid stored semen for three days and frozen-thawed semen in the north-eastern region of India. For liquid semen, the semen ejaculates were extended in Beltsville Thawing Solution (BTS) extender and preserved at 17°C for three days. For cryopreservation, semen was diluted Lactose-egg yolk-glycerol extender and frozen in straw using programmable freezer with freezing rate of 40°C/min from -6 to -140°C. The preserved evaluated for sperm motility, viability, plasma membrane integrity and fertility. The results revealed that the liquid stored semen has maintained the sperm motility and viability up to day 3 without significant reduction. Similarly the plasma membrane integrity did not differ significantly up to day 2, but it was significantly (P<0.05) reduced on days 3 in liquid stored semen. After freezing and thawing, the mean sperm motility, viability and plasma membrane integrity were 58.25 ± 2.96%, 64.75 ± 2.47% and 47.06 ± 2.02%, respectively. These parameters were significantly (P<0.01) lower as compared to the liquid stored semen from day 0 to day 3. After insemination with liquid semen, the farrowing rate was 77.7%, 80.76%, 73.07% and 69.8%, respectively from day 0, day1, day 2 and day 3. The pregnancy rate, farrowing rate and litter size did not differ significantly among different days of liquid storage. These parameters were significantly (P<0.01) lower in frozen semen as compare to that of liquid stored semen. The study concluded that the liquid semen stored up to three days is more efficient than frozen-thawed semen in terms of preserving sperm quality and fertility.
摘要译文
进行了研究以比较液体储存精液的精液质量和生育率，并在印度东北地区冷冻解冻精液。对于液体精液，在Beltsville解冻溶液（BTS）溶液中延伸精液射精，并在17℃下保存三天。对于冷冻保存，使用可编程冷冻液将精液稀释乳糖 - 蛋黄 - 甘油增量剂，并在秸秆中冷冻，从-6至-140°C的冷冻速率，冷冻速率为40℃/ min。保存的精子活性，活力，质膜完整性和生育率评价。结果表明，液体储存的精液使精子活力和活力长达第3天，而不会显着减少。类似地，血浆膜完整性没有明显差异，但在2天，但在液体储存精液中的第3天显着（p <0.05）。冷冻和解冻后，平均精子活力，活力和质子膜完整性分别为58.25±2.96％，64.75±2.47％和47.06±2.02％。与从第0天至第3天的液体储存的液体相比，这些参数显着（p <0.01）较低。在液体精液的授精后，分娩率分别为77.7％，80.76％，73.07％和69.8％ ，第1天，第2天和第3天3.怀孕率，分娩率和凋落物尺寸在液体储存的不同日子中没有显着差异。将这些参数显着（P <0.01）在冷冻精液中较低，与液体储存的精液相比。该研究得出结论，在保持精子质量和生育率方面，液体精液比储存长达三天的液体更有效。
G. Kadirvel; M. K. Kalita; Raju Kr. Dewry; Ashok Kumar; Nripendra Mahanta; L. Anandakumar Singh; S.K. Baishya;S.V. Ngachan. Semen quality and fertility of liquid stored and frozen-thawed semen in crossbred pigs of North-Eastern India[J]. Indian Journal Of Animal Research, 2019,53(1): 41-44