期刊文献

Microscopic and Molecular Identification of Eimeria Species in Domestic Rabbits (Oryctolagus cuniculus) in Romania 收藏

罗马尼亚的家兔（Oryctolagus cuniculus）中艾美酸盐物种的微观和分子鉴定

原文求助发布源

作者

Beatrice Ana-Maria Jitea (Sîrbu) (https://orcid.org/0000-0003-1536-9874) [1];Sorin Morariu (https://orcid.org/0000-0002-8814-1013) [2];Mirela Imre (https://orcid.org/0000-0003-3004-463X) [3];Tiana Florea (https://orcid.org/0000-0003-4698-4108) [4];Cătălin Bogdan Sîrbu (https://orcid.org/0000-0001-9233-7601) [5];Iasmina Luca (https://orcid.org/0000-0002-1153-6498) [6];Simona Dumitru (https://orcid.org/0000-0002-7058-4430) [7];Gheorghe Dărăbuș (https://orcid.org/0000-0002-4763-2617) [8];

作者单位

[1]Faculty of Veterinary Medicine, University of Life Sciences “King Michael I” from Timisoara, Calea Aradului 119, 300645 Timisoara, Romania; [2]Faculty of Veterinary Medicine, University of Life Sciences “King Michael I” from Timisoara, Calea Aradului 119, 300645 Timisoara, Romania; [3]Faculty of Veterinary Medicine, University of Life Sciences “King Michael I” from Timisoara, Calea Aradului 119, 300645 Timisoara, Romania; [4]Faculty of Veterinary Medicine, University of Life Sciences “King Michael I” from Timisoara, Calea Aradului 119, 300645 Timisoara, Romania; [5]Faculty of Bioengineering of Animal Resources, University of Life Sciences “King Michael I” from Timisoara, Calea Aradului 119, 300645 Timisoara, Romania; [6]Faculty of Veterinary Medicine, University of Life Sciences “King Michael I” from Timisoara, Calea Aradului 119, 300645 Timisoara, Romania; [7]Veterinary and Food Safety Department, 4 Surorile Martir Caceu, 300585 Timisoara, Romania; [8]Faculty of Veterinary Medicine, University of Life Sciences “King Michael I” from Timisoara, Calea Aradului 119, 300645 Timisoara, Romania;

发布日期

2025-4-11

页码

1109

DOI

10.3390/ani15081109

来源信息

Animals ISSN：2076-2615, 2025年, 15卷, 8期, 1109页

摘要

Coccidiosis caused by Eimeria spp. is a parasitic disease with high morbidity that affects various animal species, including rabbits. In rabbits, eleven Eimeria species have been primarily identified through microscopic examination of oocysts; however, this technique has certain limitations that make it challenging to differentiate between Eimeria species. The main objective of this study was to morphologically identify Eimeria species infecting rabbits in Romania, as well as to confirm these identifications using molecular biology techniques, specifically through the ITS-1 rRNA gene. A total of 236 samples were collected from different regions of Romania, of which 77.56% (183/236) tested positive for at least one Eimeria species. The samples were collected from rabbits raised in individual household settings, under various management systems—both in individual cages and in common ground-based enclosures. The areas of origin were chosen randomly, without applying restrictive criteria, specifically to ensure broad representativeness of the farming conditions encountered in practice, regardless of the production system or geographic specificity. The microscopic examination of morphological characteristics identified ten Eimeria species, whereas PCR results confirmed the presence of four species. It was not always possible to identify all the species present in a given sample, likely due to the predominant parasitic load and the DNA quantity of each species. The results of this study confirm that ITS-1 is a useful molecular marker for identifying Eimeria species in domestic rabbits.

摘要译文

由伊滨属引起的球虫病。是一种寄生疾病，具有高发病率，会影响包括兔子在内的各种动物物种。在兔子中，主要通过微观检查卵囊来鉴定了11种燃料。但是，这项技术具有某些局限性，使区分燃料植物的物种具有挑战性。这项研究的主要目的是在形态学上鉴定罗马尼亚感染兔子的燃料症，并使用分子生物学技术，特别是通过ITS-1 rRNA基因来确认这些鉴定。总共从罗马尼亚的不同地区收集了236个样本，其中77.56％（183/236）对至少一个艾美氏菌种类呈阳性。这些样品是从各个家庭环境中，在各种管理系统下（包括在单个笼子中的单个笼子和共同地面的围栏中）中收集的。无需应用限制标准即可随机选择原籍领域，特别是为了确保实践中遇到的农业条件的广泛代表性，无论生产系统或地理特异性如何。形态学特征的显微镜检查确定了十种嗜酸性植物，而PCR结果证实了四种物种的存在。并非总是可以识别给定样品中存在的所有物种，这可能是由于主要的寄生载荷和每个物种的DNA量所致。这项研究的结果证实，ITS-1是鉴定家养兔子中嗜酸性植物物种的有用分子标记。