期刊文献

SpHMA3: A Genetic Boost for Cadmium Tolerance and Bioremediation in Arabidopsis thaliana and Zea mays 收藏

SPHMA3：拟南芥和Zea Mays的镉耐受性和生物修复的遗传增强
原文求助 发布源
作者
Rumin Pu [1];Gaojiao Hu [2];Qian Jiang [3];Wenhao Zhou [4];Binhan Zhao [5];Chao Xia (https://orcid.org/0000-0003-1314-3644) [6];Jianfeng Hu [7];Wenqi Xiang [8];Mao Liu [9];Hanyu Deng [10];Shuang Zhao [11];Jialong Han [12];Guihua Lv [13];Haijian Lin [14];
作者单位
[1]State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, China; [2]State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, China; [3]State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, China; [4]State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, China; [5]State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, China; [6]State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, China; [7]State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, China; [8]State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, China; [9]State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, China; [10]State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, China; [11]State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, China; [12]State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, China; [13]Institute of Maize and Featured Upland Crops, Zhejiang Academy of Agricultural Sciences, Dongyang 322100, China; [14]State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, China;
发布日期
2025-4-8
页码
3487
DOI
10.3390/ijms26083487
来源信息
International Journal of Molecular Sciences ISSN：1422-0067, 2025年, 26卷, 8期, 3487页
摘要
In China, soil contamination by heavy metals is a widespread issue, with substantial increases in lead(Pb), cadmium(Cd), copper(Cu), and zinc(Zn) levels observed across various regions. Particularly, the concentrations of Pb and Cd significantly exceed their natural background levels. P-ATPases, a group of proteins, utilize energy from ATP hydrolysis to support the transmembrane movement of metal ions. This group encompasses several Heavy Metal Associated Transporter (HMA) ATPases. Studies on hyperaccumulators have shown the critical role of HMAs in the movement and reduction in Zn and Cd toxicity in plant systems. This research identifies a protein encoded by the SpHMA gene from Sedum plumbizincicola, a species noted for aiding Zn/Cd hyperaccumulators, which enhances tolerance to Cd and Zn. We detail a protein encoded by SpH/A within the HMA family that enhances Cd tolerance. Real-time fluorescence quantification (RT-PCR) indicates that SpHMA3 expression in Arabidopsis thaliana and Zea mays KN5585 correlates with high Cd tolerance, linked to Cd accumulation in Zea mays. In addition, homozygous Arabidopsis thaliana AtHMA3 mutants exhibited increased Cd sensitivity compared to the wild type (WT). Notably, plants of Arabidopsis thaliana and maize overexpressing SpHMA3 showed enhanced Cd stress tolerance compared to WT. Enhanced Cd accumulation in tissues was observed when SpHMA3 was overexpressed, as revealed by subcellular distribution analysis. We propose that SpHMA3 augments maize tolerance to Cd and Zn stresses through enhanced cellular uptake and translocation of Cd ions. This investigation clarifies the gene function of SpHMA3 in Cd and Zn stress response, offering insights for enhancing heavy metal absorption traits in maize varieties and phytoremediation methods for soils contaminated with heavy metals.
摘要译文
在中国，重金属的土壤污染是一个普遍存在的问题，铅（PB），镉（CD），铜（Cu）和锌（Zn）水平大幅增加。特别是，PB和CD的浓度显着超过其自然背景水平。P-ATPases是一组蛋白质，利用ATP水解中的能量来支持金属离子的跨膜运动。该组包括几个重金属相关转运蛋白（HMA）ATPases。关于高含量的研究表明，HMA在植物系统中Zn和CD毒性的运动和降低中的关键作用。这项研究确定了由SPHMA基因编码的蛋白质，该蛋白质是从烟雾plumbizincicola中编码的，该物种涉及辅助Zn/CD高积累剂，从而增强了对CD和Zn的耐受性。我们详细介绍了由SPH/A在HMA家族中编码的蛋白质，可增强CD耐受性。实时荧光定量（RT-PCR）表明，拟南芥和Zea mays kn5585中的Sphma3表达与高CD耐受性相关，与Zea Mays中的CD积累有关。此外，与野生型（WT）相比，纯合拟南芥thaliana athma3突变体表现出CD敏感性的提高。值得注意的是，与WT相比，拟南芥和玉米过表达的SPHMA3的植物表现出增强的CD胁迫耐受性。如下细胞分布分析所揭示的，当SPHMA3过表达时，观察到组织中的CD积累增强。我们建议SPHMA3通过增强的细胞摄取和CD离子易位来增强玉米对CD和Zn应力的耐受性。这项研究阐明了CD和Zn应激反应中SPHMA3的基因功能，提供了增强玉米品种中重金属吸收特征的见解和对被重金属污染的土壤的植物修复方法。
Rumin Pu [1];Gaojiao Hu [2];Qian Jiang [3];Wenhao Zhou [4];Binhan Zhao [5];Chao Xia (https://orcid.org/0000-0003-1314-3644) [6];Jianfeng Hu [7];Wenqi Xiang [8];Mao Liu [9];Hanyu Deng [10];Shuang Zhao [11];Jialong Han [12];Guihua Lv [13];Haijian Lin [14];. SpHMA3: A Genetic Boost for Cadmium Tolerance and Bioremediation in Arabidopsis thaliana and Zea mays[J]. International Journal of Molecular Sciences, 2025,26(8): 3487