摘要
【Abstract】 Two methods were compared for their ability to accurately identify Vibrio species of interest: whole genome sequencing as the reference method and MALDI-TOF MS (matrix-assisted laser desorption/ionization-time of flight mass spectrometry) proteome fingerprinting. The accuracy of mass spectrometry–based identification method was evaluated for its ability to accurately identify isolates of Vibrio cholerae and Vibrio parahaemolyticus. Identification result of each isolate obtained by mass spectrometry was compared to identification by whole genome sequencing (WGS). The MALDI-TOF MS system had excellent performance for identification of V. cholerae and V. parahaemolyticus isolates grown on a non-selective solid agar media. Unlike the biochemical characterization performed by API20E. In this study, 161 isolates (V. cholerae, n = 33; V. parahaemolyticus, n = 102; V. spp., n = 23; other enteropathogens, Salmonella and E. coli, n = 3) were used to assess accuracy. The MALDI-TOF MS system was able to accurately identify 100% (33/33) of the V. cholerae isolates and 99.9% (101/102) of V. parahaemolyticus isolates, with 100% for both sensitivity and specificity for V. cholerae and 99% sensitivity and 98% specificity for V. parahaemolyticus. Thus, mass spectrometry for bacterial identification is comparable to the WGS. Furthermore, in comparison to a biochemical characterization, the use of MALDI-TOF MS system shortens the analysis time from over 72 h to less than 24 h. 【Key points】 • V. cholerae and V. parahaemolyticus were successfully ID-ed by MALDI-TOF • MALDI-TOF sensitivity and specificity parallels the WGS method of identification • MALDI-TOF is several days faster than the battery of culture-dependent methods
摘要译文
【摘要】将两种方法的准确识别感兴趣的弧菌物种进行了比较：整个基因组测序作为参考方法和MALDI-TOF MS（矩阵辅助激光解吸/飞行质谱的电离时间）蛋白质组指纹打印。评估了基于质谱法的鉴定方法的准确性，其能够准确鉴定纤维霍乱和弧菌性副溶血性的分离株的能力。将通过质谱法获得的每个分离株的鉴定结果与整个基因组测序（WGS）进行了比较。MALDI-TOF MS系统具有出色的性能，可鉴定在非选择性固体琼脂培养基上生长的霍乱弧菌和副溶血分离株。与API20E进行的生化表征不同。在这项研究中，使用161个分离株（V. cholerae，n = 33; V. parahayticus，n = 102; V. spp。，n = 23;其他肠病毒，沙门氏菌和大肠杆菌，n = 3）来评估准确性。MALDI-TOF MS系统能够准确识别霍乱弧菌分离株的100％（33/33）和99.9％（101/102）V。parahayticus ryase株，对V.霍乱的敏感性和特异性为100％对副溶血杆菌的敏感性和98％的敏感性和98％的特异性。因此，细菌鉴定的质谱法与WGS相当。此外，与生化特征相比，使用MALDI-TOF MS系统的使用将分析时间从72小时以上缩短到不到24小时。【关键点】•V。霍乱和V. parahayticus成功地通过Maldi-Tof•Maldi-TOF敏感性和特异性与WGS识别方法相似•MALDI-TOF比培养依赖性方法快几天了
Swapan Banerjee [1];Annika Flint [2];Madeleine B. Brosseau [3];Kelly Weedmark [4];Bojan Shutinoski (https://orcid.org/0000-0003-4669-5656) [5];. Evaluation of MALDI-TOF for identification of Vibrio cholerae and Vibrio parahaemolyticus from growth on agar media[J]. Applied Microbiology and Biotechnology, 2025,109(1): 5