摘要
The purpose of this study was to investigate lactic acid bacteria with antioxidative and probiotic activities isolated from Korean healthy infant feces and kimchi. Isolates A1, A2, S1, S2, and S3 were assigned to Lactobacillus sp. and isolates A3, A4, E1, E2, E3, and E4 were assigned to Leuconostoc sp. on the basis of their physiological properties and 16S ribosomal DNA sequence analysis. Most strains were confirmed as safe bioresources through nonhemolytic activities and non-production of harmful enzymes such as β-glucosidase, β-glucuronidase and tryptophanase. The 11 isolates showed different resistance to acid and bile acids. In addition, they exhibited antibacterial activity against foodborne bacteria, especially Bacillus cereus, Listeria monocytogenes, and Escherichia coli. Furthermore, all strains showed significantly high levels of hydrophobicity. The antioxidant effects of culture filtrates of the 11 strains included 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging capacity, 2.2'-azino-bis (2-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical cation scavenging activity, and superoxide dismutase activity. The results revealed that most of the culture filtrates have effective scavenging activity for DPPH and ABTS radicals. All strains appeared to have effective superoxide dismutase activity. In conclusion, the isolated strains A1, A3, S1, and S3 have significant probiotic activities applicable to the development of functional foods and health-related products. These strains might also contribute to preventing and controlling several diseases associated with oxidative stress, when used as probiotics.
摘要译文
这项研究的目的是调查从韩国健康的婴儿粪便和泡菜中分离出的具有抗氧化和益生菌活性的乳酸菌。分离株A1，A2，S1，S2和S3被分配给乳酸杆菌属。并将分离物A3，A4，E1，E2，E3和E4分配给Leuconostoc sp。根据它们的生理特性和16S核糖体DNA序列进行分析。通过非溶血活性和不产生有害酶（例如β-葡萄糖苷酶，β-葡萄糖醛酸苷酶和色氨酸酶），大多数菌株被确认为安全的生物资源。这11种分离株对酸和胆汁酸表现出不同的抗性。此外，它们还对食源性细菌，尤其是蜡样芽孢杆菌，单核细胞增生性李斯特菌和大肠杆菌具有抗菌活性。此外，所有菌株均显示出显着高水平的疏水性。 11个菌株的培养滤液的抗氧化作用包括清除2,2-二苯基-1-吡啶基-肼基（DPPH）自由基，2.2'-叠氮基-双（2-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基阳离子清除活性和超氧化物歧化酶活性。结果表明，大多数培养滤液对DPPH和ABTS自由基具有有效的清除活性。所有菌株似乎都具有有效的超氧化物歧化酶活性。总之，分离出的菌株A1，A3，S1和S3具有重要的益生菌活性，可用于开发功能性食品和健康相关产品。当用作益生菌时，这些菌株还可能有助于预防和控制与氧化应激有关的多种疾病。
Ji; Keunho;Jang; Na Young;Kim; Young Tae;. Isolation of Lactic Acid Bacteria Showing Antioxidative and Probiotic Activities from Kimchi and Infant Feces[J]. Journal of Microbiology and Biotechnology, 2015,25(9): 1568-1577