摘要
Superovulated Jersey and Holstein heifers and cows were bred 9.7 ± 2.7 h after the first observation of estrus with a single dose of frozen semen. Animals were grouped by site of insemination: 1) right uterine horn (n = 5), 2) left uterine horn (n = 4), 3) mid- uterine body (n = 5), and 4) mid-cervix (n = 6). The number of unfertilized ova, normal and abnormal embryos were recorded for each horn at slaughter 115.8 ± 18.6 h after insemination. All viable embryos were cultured in vitro and assessed for development. The overall fertilization rate was 76.8%, with the ipsilateral horn being higher than that of the contralateral horn (P 0.05). Under the conditions of this study with superovulated cows, these results suggest than 1) spermatozoa migrate from one horn to the other and 2) inseminating too deep into one horn may reduce the chances for concention when ovulation occurs contralaterally.
摘要译文
在用单剂量冷冻精液首次观察发情后，将超排卵泽西和荷斯坦母牛和奶牛饲养9.7±2.7小时。根据授精位点对动物进行分组：1）右子宫角（n = 5），2）子宫角（n = 4），3）子宫体（n = 5），和4）子宫颈中段（n） = 6）。在授精后115.8±18.6 h屠宰时，记录每个角的未受精卵，正常和异常胚胎的数量。在体外培养所有存活的胚胎并评估其发育。总受精率为76.8％，同侧角高于对侧角（P <0.05）。在超排卵母牛的这项研究条件下，这些结果表明，1）精子从一个角落迁移到另一个角落; 2）过度深入一个角落可能会减少对侧排卵时集中的机会。
A. Pallares P.M. Zavos R.W. Hemken. Fertilization rates and embryonic development in superovulated cattle inseminated in different sites within the reproductive tract[J]. Theriogenology, 1986,26(6): 709-719