期刊文献

Structure and Function Analyses of the Thioredoxin 2 and Thioredoxin Reductase Gene in Pacific White Shrimp (Litopenaeus vannamei) 收藏

太平洋白虾（Litopenaeus vannamei）中硫氧还蛋白2和硫氧还蛋白还原酶基因的结构和功能分析

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作者

作者单位

[1]Hainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bio-Resources, Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China; [2]Hainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bio-Resources, Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China; [3]Hainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bio-Resources, Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China; [4]Hainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bio-Resources, Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China; [5]Hainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bio-Resources, Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China; [6]Hainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bio-Resources, Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China; [7]Hainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bio-Resources, Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China; [8]College of Biology and Agriculture, Jiamusi University, Jiamusi 154007, China; [9]Hainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bio-Resources, Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China; [10]Hainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bio-Resources, Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China;

发布日期

2025-2-21

页码

629

DOI

10.3390/ani15050629

来源信息

Animals ISSN：2076-2615, 2025年, 15卷, 5期, 629页

摘要

The thioredoxin (Trx) system is one of the most significant systems in living organisms as it regulates cellular redox reactions and plays a pivotal protective role within the cell by promoting redox homeostasis. Trx and thioredoxin reductase (TrxR) are the core oxidoreductases of the Trx system. In this study, the novel full-length cDNAs of LvTrx2 and LvTrxR were cloned from Litopenaeus vannamei. The ORFs of LvTrx2 and LvTrxR were 453 bp and 1785 bp, encoding polypeptides consisting of 150 and 596 amino acids. Sequence alignment analysis revealed that the amino acid sequence of LvTrx2 shared a high degree of identity (93%) with that of Penaeus chinensis, while in LvTrxR, it exhibited a similarity level of 95% with previously submitted Penaeus chinensis and Penaeus monodon sequences. Regarding tissue-specific expression patterns, LvTrx2 showed its highest expression levels in hepatopancreas and gill. For LvTrxR, the highest expression was observed in gill followed by hepatopancreas and intestine. During exposure to ammonia-N, there was a significant upregulation in the relative mRNA levels of LvTrx2 and LvTrxR in hepatopancreas and gill, with the peak values occurring at 24 h or 48 h of exposure. After LPS injection, the LvTrx2 and LvTrxR transcripts in hepatopancreas and gill had different upregulated levels. These findings suggest that LvTrx2 and LvTrxR play pivotal roles in enhancing stress resistance and bolstering antibacterial defense mechanisms in L. vannamei. To explore the roles, LvTrx2 expression was knocked down in vivo to verify the defense mechanism against 4-NP stress. LvTrx2 silencing in 4-NP-challenged shrimp could significantly induce the gene expression of antioxidant-related genes (except for LvTrxR) and aggravate the oxidative damage of lipids. This study suggests that the Trx system is involved in regulating the antioxidant processes, and LvTrx2 and LvTrxR play a vital role in defense responses against environmental stress.

摘要译文

硫氧还蛋白（TRX）系统是生物体中最重要的系统之一，因为它调节细胞氧化还原反应，并通过促进氧化还原稳态在细胞中起关键的保护作用。TRX和硫氧还蛋白还原酶（TRXR）是TRX系统的核心氧化还原酶。在这项研究中，从Litopenaeus Vannamei克隆了LVTRX2和LVTRXR的新型全长cDNA。LVTRX2和LVTRXR的ORF为453 bp和1785 bp，编码由150和596个氨基酸组成的多肽。序列比对分析表明，LVTRX2的氨基酸序列与Penaeus Chinensis具有高度的身份（93％），而在LVTRXR中，它与先前提交的Penaeus Chinensis和Penaeus Monodon序列相似，为95％。关于组织特异性的表达模式，LVTRX2在肝癌和Gill中显示出最高的表达水平。对于LVTRXR，在吉尔中观察到最高的表达，其次是肝癌和肠道。在暴露于氨-N的过程中，在肝癌和gill中，LVTRX2和LVTRXR的相对mRNA水平显着上调，峰值在暴露时24 h或48 h发生。注射LPS后，肝癌和吉尔中的LVTRX2和LVTRXR转录本具有不同的上调水平。这些发现表明，LVTRX2和LVTRXR在增强抗压力性和加强Vannamei的抗菌防御机制方面起着关键作用。为了探索角色，LVTRX2表达在体内被击倒，以验证针对4-NP胁迫的防御机制。4-NP挑战的虾中的LVTRX2沉默可以显着诱导抗氧化剂相关基因的基因表达（LVTRXR除外），并加剧脂质的氧化损伤。这项研究表明，TRX系统参与调节抗氧化剂过程，LVTRX2和LVTRXR在针对环境压力的防御反应中起着至关重要的作用。

Tong Xu [1];Pei-Hua Zheng [2];Ke-Er Luan [3];Xiu-Xia Zhang [4];Jun-Tao Li (https://orcid.org/0009-0002-8619-1364) [5];Ze-Long Zhang [6];Wei-Yan Hou [7];Li-Min Zhang [8];Yao-Peng Lu [9];Jian-An Xian (https://orcid.org/0000-0001-6026-8189) [10];. Structure and Function Analyses of the Thioredoxin 2 and Thioredoxin Reductase Gene in Pacific White Shrimp (Litopenaeus vannamei)[J]. Animals, 2025,15(5): 629