摘要
The v‐erbB gene is an oncogene of the avian erythroblastosis virus encoding a protein that is a truncated version of the epidermal growth factor receptor. The v‐erbB protein was expressed alone or as polyhedrin‐erbB fusion proteins using the Bombyx mori nuclear polyhedrosis virus vector. The expression level of the fusion protein whose polyhedrin portion consisted of only 8 amino‐terminal amino acids was more than ten times higher than that of the non‐fusion protein. Studies with tunkamycin showed that the recombinant v‐erbB proteins were glycosylated. The recombinant protein autophosphorylated tyrosine residues, and phosphorylated a synthetic tyrosine‐containing peptide and lipocortin I. These observations indicate that functional v‐erbB protein can he expressed in silkworm‐derived cells, and furthermore, that this system can be used for large‐scale production.
摘要译文
V -ERBB基因是编码表皮生长因子受体的截断版本的蛋白质的禽红细胞病毒的癌基因。V -ERBB蛋白单独表达或使用Bombyx Mori核多面病毒病毒载体作为多面蛋白-ERBB融合蛋白。融合蛋白的表达水平仅由8个氨基末端氨基酸组成，比非灌注蛋白的表达水平高10倍以上。对隆霉素的研究表明，重组V -ERBB蛋白是糖基化的。重组蛋白自动磷酸酪氨酸残基，并磷酸化了含有合成酪氨酸的肽和脂皮质素I。这些观察表明，他可以在丝虫衍生的细胞中表达功能性V -ERBB蛋白，并且该系统可用于大型尺度尺度的大小尺度。生产。
Kaoru Morishita; Masahiro Iwamoto; Kenji Murakami; Minoru Kubota; Susumu Maeda; Kumao Toyoshima; ; Tadashi Yamamoto. Expression and Characterization of Kinase‐active v‐erbB Protein Using a Baculovirus Vector System[J]. Japanese Journal of Cancer Research GANN, 1992,83(1): 52-60