摘要
In a previously reported genome-wide association study based on a high-density bovine SNP genotyping array, 8 SNP were nominally associated (P ≤ 0.003) with average daily gain (ADG) and 3 of these were also associated (P ≤ 0.002) with average daily feed intake (ADFI) in a population of crossbred beef cattle. The SNP were clustered in a 570 kb region around 38 Mb on the draft sequence of bovine chromosome 6 (BTA6), an interval containing several positional and functional candidate genes including the bovine LAP3, NCAPG, and LCORL genes. The goal of the present study was to develop and examine additional markers in this region to optimize the ability to distinguish favorable alleles, with potential to identify functional variation. Animals from the original study were genotyped for 47 SNP within or near the gene boundaries of the three candidate genes. Sixteen markers in the NCAPG-LCORL locus displayed significant association with both ADFI and ADG even after stringent correction for multiple testing (P ≤ 005). These markers were evaluated for their effects on meat and carcass traits. The alleles associated with higher ADFI and ADG were also associated with higher hot carcass weight (HCW) and ribeye area (REA), and lower adjusted fat thickness (AFT). A reduced set of markers was genotyped on a separate, crossbred population including genetic contributions from 14 beef cattle breeds. Two of the markers located within the LCORL gene locus remained significant for ADG (P ≤ 0.04). Several markers within the NCAPG-LCORL locus were significantly associated with feed intake and body weight gain phenotypes. These markers were also associated with HCW, REA and AFT suggesting that they are involved with lean growth and reduced fat deposition. Additionally, the two markers significant for ADG in the validation population of animals may be more robust for the prediction of ADG and possibly the correlated trait ADFI, across multiple breeds and populations of cattle.
摘要译文
在先前报道的基于高密度牛SNP基因分型阵列的基因组 - 宽协会研究中，8个SNP标称相关（P≤0.003），平均每日增益（ADG），其中3个也相关（P≤0.002）跨碎牛肉牛群的平均每日饲料摄入量（ADFI）。在牛染色体6（BTA6）的草稿序列中，将SNP聚集在约38 MB的570 kB区域中，含有若干位置和功能候选基因，包括牛LAP3，NCAPG和LCORL基因的间隔。本研究的目标是在该地区开发和检查其他标记，以优化区分有利等位基因的能力，潜力识别功能变异。来自原始研究的动物在三个候选基因的基因边界内或附近的47个SNP进行基因分型。即使在对多次测试的严格校正后，NCAPG-LCORL基因座中的十六个标记也与ADFI和ADG显示出显着的关联（P≤005）。评估这些标记对肉和胴体性状的影响。与较高ADFI和ADG相关的等位基因也与较高的热胎体重量（HCW）和Ribeye区域（REA）相关，以及更低的调节脂肪厚度（AFT）。一组减少的标记物在一个单独的杂交群体中基因分型，包括来自14种牛肉群的遗传贡献。位于LCORL基因基因座内的两个标记对于ADG而言仍有显着性（P≤0.04）。 NCAPG-LCORL基因座内的几个标记与进料摄入和体重增加表型显着相关。这些标志物还与HCW，Rea和Aft相关的，表明它们参与贫生长和减少脂肪沉积。另外，对于动物验证群体中ADG的两个标记对于预测ADG和可能的具有相关性状ADFI，涉及多种品种和牛群的患者可能更加稳健。
Amanda K Lindholm-Perry; Andrea K Sexten; Larry A Kuehn; Timothy PL Smith; D Andy King; Steven D Shackelford; Tommy L Wheeler; Calvin L Ferrell; Thomas G Jenkins; Warren M Snelling;Harvey C Freetly. Association, effects and validation of polymorphisms within the NCAPG - LCORL locus located on BTA6 with feed intake, gain, meat and carcass traits in beef cattle[J]. BMC Genomic Data, 2011,12