期刊文献

Fertilization rate in sheep unilaterally inseminated with frozen semen 收藏

绵羊单侧受精冷冻精液的受精率

原文求助发布源

作者

J.E.Correa;B.Bergmann;R.Gatica;

作者单位

Animal Reproduction Institute, Faculty of Veterinary Sciences, Universidad Austral de Chile, P.O. Box 567, Valdivia, Chile

关键词

Sheep; Artificial insemination; Frozen semen; Fertilization rate; Surgical technique;

关键词译文

羊;人工授精;冷冻精液;施肥率;手术技术;

发布日期

1 January 1994

页码

99-101

DOI

10.1016/0921-4488(94)90037-X

来源信息

Small Ruminant Research ISSN：0921-4488, 1994年, 13卷, 1期, 99-101页

摘要

In order to evaluate a simple surgical technique for artificial insemination (AI) with frozen semen, 40 adult ewes were synchronised with vaginal sponges impregnated with 60 mg of medroxyprogesterone acetate for 12 d and a single injection of 400 IU PMSG at sponge withdrawal. Semen from fertile rams was pooled and frozen in straws, each containing 3 × 10⁸ spermatozoa. All ewes were inseminated 60 h after PMSG injection. Twenty ewes (group A) were unilaterally inseminated by a small midventral laparotomy and 20 (group B) were cervically inseminated. Fertilization rate was measured by flushing of uteri and oviducts throughout a laparotomy carried out 3–4 d after insemination. The 83.7% fertilization rate in group A was significantly different (P

摘要译文

为了评估用冷冻精液进行人工授精（AI）的简单手术技术，将40只成年母羊与用60mg醋酸甲羟孕酮浸渍12天的阴道海绵同步，并在海绵戒断时单次注射400IU PMSG。将来自肥沃公羊的精液合并并用吸管冷冻，每个吸管含有3×10 ^{8 精子。在PMSG注射后60小时，所有母羊都被授精。 20只母羊（A组）通过小型腹腔切开术单侧受精，20只（B组）进行宫颈受精。通过在授精后3-4天进行的剖腹手术中通过冲洗子宫和输卵管来测量受精率。 A组受精率为83.7％，差异显着（P <0.05）}

J.E.Correa;B.Bergmann;R.Gatica;. Fertilization rate in sheep unilaterally inseminated with frozen semen[J]. Small Ruminant Research, 1994,13(1): 99-101