期刊文献

Oral supplementation with Scabiosa artropurperea L. aqueous extract improves the in vivo sexual behaviour, sperm quality and androgen level in rams 收藏

用scabiosa artropurperea L.口腔补充水。

原文求助发布源

作者

作者单位

[1]Université de Jendouba, Ecole Supérieure d'Agriculture du Kef, Laboratoire d'Appui à la Durabilité des Systèmes de Production Agricole dans la Région du Nord‐Ouest ESAK Kef Tunisia; [2]Université de Jendouba, Ecole Supérieure d'Agriculture du Kef, Laboratoire d'Appui à la Durabilité des Systèmes de Production Agricole dans la Région du Nord‐Ouest ESAK Kef Tunisia; [3]Université de Jendouba, Ecole Supérieure d'Agriculture du Kef, Laboratoire d'Appui à la Durabilité des Systèmes de Production Agricole dans la Région du Nord‐Ouest ESAK Kef Tunisia; [4]ADIPARA, Ecole Supérieure d'Agriculture de Mateur University of Carthage Carthage Tunisia; [5]Université de Jendouba, Ecole Supérieure d'Agriculture du Kef, Laboratoire d'Appui à la Durabilité des Systèmes de Production Agricole dans la Région du Nord‐Ouest ESAK Kef Tunisia; [6]CNRS, IFCE, INRAE, Université de Tours, PRC Nouzilly France; [7]Laboratory of Clinical Biochemistry Charles Nicolle Hospital Tunis Tunisia; [8]Laboratory of Clinical Biochemistry Charles Nicolle Hospital Tunis Tunisia; [9]CNRS, IFCE, INRAE, Université de Tours, PRC Nouzilly France; [10]CNRS, IFCE, INRAE, Université de Tours, PRC Nouzilly France; [11]Université de Jendouba, Ecole Supérieure d'Agriculture du Kef, Laboratoire d'Appui à la Durabilité des Systèmes de Production Agricole dans la Région du Nord‐Ouest ESAK Kef Tunisia;

发布日期

2024-5-7

DOI

10.1111/rda.14569

来源信息

Reproduction in Domestic Animals ISSN：0936-6768, 2024年, 59卷, 5期

摘要

【Abstract】The effects of an aqueous extract of Scabiosa atropurpurea L. (AES) on the reproduction potential of Queue Fine de l'Ouest rams were evaluated over 9 weeks. Eighteen mature (4–6 years old) rams (52.8 ± 2.6 kg) were divided into three groups. The control (C) group was fed oat hay ad libitum with 700 g of concentrate and the other two groups were fed the same diet supplemented with AES at 1 and 2 mg/kg body weight (AES1 and AES2, respectively). Ram sperm was collected with an artificial vagina (2 × 2 days/week) to evaluate sperm production and quality, antioxidant activity, the adenosine triphosphate (ATP) and calcium concentrations. Sexual behaviour and plasma testosterone concentrations were also investigated. The administration of AES improved sexual behaviour (the duration of contact and the number of lateral approaches). The addition of AES also improved individual spermatozoa motility (C: 71.7% ± 6.3%; AES1: 78.3% ± 4.9%; AES2: 83.8% ± 4.4%), the sperm concentration (C: 5.6 ± 0.36; AES1: 6.4 ± 0.81; AES2: 6.7 ± 0.52 × 109 spermatozoa/mL), the ATP ratio (C: 1 ± 0.08; AES1: 2.1 ± 0.08; AES2: 3.3 ± 0.08) and the calcium concentration (C: 5.6 ± 0.24; AES1: 7.7 ± 0.21; AES2: 8.1 ± 0.24 mmol/L). AES treatment decreased the percentage of abnormal sperm (C: 18.5% ± 1.2%; AES1: 16.2% ± 1.1%; AES2: 14.8% ± 0.94%) and DNA damage (C: 62%; AES1: 27%; AES2: 33%) and was associated with elevated seminal fluid antioxidant activity (C: 22 ± 0.27; AES1: 27.1 ± 1.08 and AES2: 27.5 ± 0.36 mmol Trolox equivalents/L) and plasma testosterone (C: 8.3 ± 0.7; AES1: 11.7 ± 0.4; AES2: 15 ± 0.7 ng/L). In conclusion, our study suggests that S. atropurpurea may be potentially useful to enhance libido and sperm production and quality in ram.

摘要译文

【摘要】在9周内评估了scabiosa adpurpurea L.（AES）水提取物对队列细胞的繁殖潜力的影响。将十八个成熟（4-6岁）的公羊（52.8±2.6 kg）分为三组。对照组（c）组为燕麦干草，浓缩物为700 g，另外两组则以1和2 mg/kg体重（分别为AES1和AES2）喂食相同的AES饮食。用人造阴道（2×2天/周）收集RAM精子，以评估精子的产生和质量，抗氧化活性，三磷酸腺苷（ATP）和钙浓度。还研究了性行为和血浆睾丸激素浓度。AE的给药改善了性行为（接触时间和横向方法的数量）。AES的添加还提高了个体精子运动性（C：71.7％±6.3％； AES1：78.3％±4.9％； AES2：83.8％±4.4％），精子浓度（C：5.6±0.36; AES1：6.4±0.81; 6.4±0.81±0.81; AES2：6.7±0.52×109精子/ml），ATP比（C：1±0.08; AES1：2.1±0.08; AES2：AES2：3.3±0.08）和钙浓度0.21; AES2：8.1±0.24 mmol/l）。AES治疗降低了异常精子的百分比（C：18.5％±1.2％； AES1：16.2％±1.1％； AES2：14.8％±0.94％）和DNA损害（C：62％； AES1：27％; AES2：33：33：33：33：33：33：33％），并且与升高的精液液抗氧化剂活性有关（C：22±0.27; AES1：27.1±1.08和AES2：27.5±0.36 mmol trolox等效剂/L）和血浆睾丸激素（C：8.3±0.7; AES1：11.7±0.4; AES1：11.7±0.4±0.4±0.4±0.4±0.4±0.4;AES2：15±0.7 ng/l）。总而言之，我们的研究表明，促链霉菌链球菌可能对增强RAM中的性欲和精子产生和质量有可能有用。

Wijden Niama [1];Samia Ben Saïd (0000-0003-1760-7127) [2];Mohamed Aroua [3];Sihem Amiri (0000-0003-4780-9758) [4];Hnia Chograni [5];Christelle Ramé [6];Kahena Bouzid [7];Ahlem Bartakiz [8];Pascal Froment [9];Joelle Dupont [10];Mokhtar Mahouachi [11];. Oral supplementation with Scabiosa artropurperea L. aqueous extract improves the in vivo sexual behaviour, sperm quality and androgen level in rams[J]. Reproduction in Domestic Animals, 2024,59(5)