摘要
Cold carcass weight (CW) and longissimus muscle area (EMA) are the major quantitative traits in beef cattle. In this study, we found several polymorphisms of growth hormone-releasing hormone (GHRH) gene and examined the association of polymorphisms with carcass traits (CW and EMA) in Korean native cattle (Hanwoo). By direct DNA sequencing in 24 unrelated Korean cattle, we identified 12 single nucleotide polymorphisms within the 9 kb full gene region, including the 1.5 kb promoter region. Among them, six polymorphic sites were selected for genotyping in our beef cattle (n = 428) and five marker haplotypes (frequency > 0.1) were identified. Statistical analysis revealed that -4241A>T showed significant associations with CW and EMA. Our findings suggest that polymorphisms in GHRH might be one of the important genetic factors that influence carcass yield in beef cattle. Sequence variation/haplotype information identified in this study would provide valuable information for the production of a commercial line of beef cattle.
摘要译文
冷胴体重量（CW）和肌肉肌肉区域（EMA）是牛肉中的主要定量性状。在这项研究中，我们发现了几种生长激素释放激素（GHRH）基因的多态性，并检查了韩国天然牛（HANWOO）中与胴体性状（CW和EMA）的多态性的关联。通过在24个无关的韩国牛中直接DNA测序，我们在9kb全基因区域内鉴定了12个单核苷酸多态性，包括1.5kb启动子区域。其中，选择六种多态性位点进行基因分型在我们的牛粪（n = 428）中，并鉴定了五个标记单倍型（频率> 0.1）。统计分析显示 - 4241A> T显示出与CW和EMA的显着关联。我们的研究结果表明，GHRH中的多态性可能是影响肉牛中胴体产量的重要遗传因素之一。本研究中确定的序列变异/单倍型信息将为生产商业线路的商业线路提供有价值的信息。
Hyun Sub Cheong; Du-Hak Yoon; Lyoung Hyo Kim; Byung Lae Park; Yoo Hyun Choi; Eui Ryong Chung; Yong Min Cho; Eng Woo Park; Il-Cheong Cheong; Sung-Jong Oh; Sung-Gon Yi; Taesung Park;Hyoung Doo Shin. Growth hormone-releasing hormone (GHRH) polymorphisms associated with carcass traits of meat in Korean cattle[J]. BMC Genomic Data, 2006,7