摘要
【ABSTRACT】Human interference reduces wild felid populations. Somatic cell nuclear transfer and the use of induced pluripotent stem cells are potential conservation strategies. To improve the efficiency of these strategies, it is essential to establish adequate protocols for the synchronization of cells in the G0/G1 phase of the cell cycle. Cell cycle synchronization can arrest cell cycle progression by inhibiting factors involved in cell duplication. However, this step varies among wild felids and has not been successful in some species. In addition, the effect of this step on cell applications remains unclear. Therefore, this review highlights the primary differences among wild felids that can cause this variability, the most promising results, and the methods used. Finally, the importance of cell cycle synchronization in biotechnologies involving the nuclear reprogramming of somatic cells in wild felid conservation is highlighted.
摘要译文
【摘要】人类干扰减少了野生的FELID种群。体细胞核转移和诱导多能干细胞的使用是潜在的保护策略。为了提高这些策略的效率，必须在细胞周期的G0/G1阶段建立足够的方案来同步细胞。细胞周期同步可以通过抑制细胞重复的因素来阻止细胞周期进展。但是，这一步骤在野生猫咪之间有所不同，并且在某些物种中没有成功。此外，此步骤对细胞应用的影响尚不清楚。因此，本综述突出了野生猫咪之间的主要差异，这些野生动物可能导致这种可变性，最有希望的结果以及所使用的方法。最后，强调了细胞周期同步在涉及野生FELID保护中体细胞核重编程的生物技术中的重要性。
João V.S. Viana [1];Alexsandra F. Pereira (https://orcid.org/0000-0003-2137-854X) [2];. The Role of Somatic Cell Synchronization in Nuclear Transfer and Induced Pluripotent Stem Cells for Wild Felids[J]. Zoo Biology, 2025,44(3): 221-229