摘要
【Abstract.】 The capacity of adult rat testes rendered surgically cryptorchid to metabolize progesterone1 was investigated at several time intervals (7, 14, 28 and 42 days) after surgery. Testicular tissue was incubated with [3H]progesterone, the steroid metabolites were isolated and purified using paper and thin layer chromatography, and further identified by isotopic dilution and recrystallization to constant 3H/14C ratio or constant specific activity (dpm/μmol). A sharp decrease in the accumulation of testosterone, concomitant with a marked increase in accumulation of 17α-hydroxyprogesterone, was observed as early as 7 days after surgery. No further major changes were observed at 14 or 28 days after surgery. However, at 42 days, accumulation of testosterone was substantially increased, while that of 17α-hydroxyprogesterone decreased to trace levels. 20α-Dihydroprogesterone was detected at different time intervals after surgery. The data suggest that cryptorchidism inhibits the activity of 17–20 desmolase for the first 4 weeks after surgery. The activity of this enzyme is restored by the 42nd day and the metabolic pathway between progesterone and testosterone resembles that of an intact rat. The factors responsible for this spontaneous restoration of the biosynthetic pathway are under investigation.
摘要译文
【摘要。】在手术后几个时间间隔（7、14、28和42天）研究了由手术加密的成年大鼠睾丸的能力。将睾丸组织与[3H]孕酮孵育，分离并用纸和薄层色谱法分离并纯化类固醇代谢产物，并通过同位素稀释和重结晶到恒定的3H/14C比例或恒定比率或恒定特异性活性（DPM/μmol）。睾丸激素的积累急剧下降，与术后7天后观察到17α-羟基酸蛋白酶的积累显着增加。手术后14或28天未观察到进一步的重大变化。然而，在42天的时间里，睾丸激素的积累大大增加，而17α-羟基苯甲酸酯的积累降低到痕量水平。手术后，以不同的时间间隔检测到20α-二氢雌激素。数据表明，密码术在手术后的头4周抑制17-20脱糖酶的活性。该酶的活性在第42天恢复，孕酮和睾丸激素之间的代谢途径类似于完整的大鼠。造成生物合成途径的自发恢复的因素正在研究中。
Miguel Ficher [1];Emil Steinberger [2];. Conversion of progesterone by testicular tissue of cryptorchid rats[J]. European Journal of Endocrinology, 1982,101(2): 301–306