摘要
The present study was designed to investigate the possible modulator effect of melatonin on uterine estrogen and progesterone receptors in rats as well as the uterine response to oxytocin. Non-pregnant rats were pretreated with melatonin in a dose of 0.8 mg kg−1 per day for 15 consecutive days. Control animals received the vehicle. The uterus was dissected out and uterine contraction in one horne was recorded in vitro for each animal as a response to oxytocin (0.5×10−11 to 2×10−11 M). The other uterine horne was subjected to estrogen and progesterone receptors detection by immunohistochemical and image analysis techniques. The results reveal a significant reduction (59%) in the number of uterine estrogen receptors with concomitant increase in the progesterone receptors (53%) in melatonin-pretreated rats as compared to the control ones. In addition, our data show an inhibitory effect of melatonin on the uterine contraction as a response to oxytocin (0.5×10−11, 1×10−11, and 2×10−11 M) amounting to 48, 77, and 59.5% reduction, respectively, in the amplitude of contraction as well as 62, 19.9, and 47% reduction in the area under the curve (AUC) of uterine contractions, respectively. The data, so far obtained, may indicate a possible relationship of melatonin-induced modulation of the number of estrogen and progesterone receptors and its inhibitory effect on uterine contraction. These findings merit further investigations on the possible beneficial role of melatonin in a plethora of hormone-dependant uterine disorders.
摘要译文
本研究旨在探讨褪黑素对大鼠子宫雌激素和孕激素受体的可能调节作用以及子宫对催产素的反应。非挽救大鼠用褪黑激素以0.8mg / kg -1 每天的剂量预处理，连续15天。对照动物接受载体。解剖子宫并在体外记录每只动物的一个角的子宫收缩作为对催产素的反应（0.5×10 -11 至2×10 -11 M ）。通过免疫组织化学和图像分析技术检测另一个子宫角的雌激素和孕酮受体。结果显示，与对照组相比，褪黑素预处理大鼠的子宫雌激素受体数量显着减少（59％），伴随黄体酮受体（53％）的增加。此外，我们的数据显示褪黑素对子宫收缩的抑制作用是对催产素的反应（0.5×10 -11 ，1×10 -11 和2× 10 -11 M）收缩幅度分别减少48％，77％和59.5％，曲线下面积减少62％，19.9％和47％（AUC）分别为子宫收缩迄今获得的数据可能表明褪黑激素诱导的雌激素和孕酮受体数量调节的可能关系及其对子宫收缩的抑制作用。这些发现值得进一步研究褪黑激素在多种激素依赖性子宫疾病中的可能有益作用。
Adel R.AAbd-Allah;El-Sayed MEl-Sayed;Mohamed HAbdel-Wahab;Farid M.AHamada;. Effect of melatonin on estrogen and progesterone receptors in relation to uterine contraction in rats[J]. Pharmacological Research, 2003,47(4): 349-354